Cultural characteristics
Biochemical characters
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Enterococcaceae, Genus Enterococcus, Enterococcus thailandicus
Tanasupawat et al. 2008.
According to Shewmaker Patricia Lynn et al. 2011,
Enterococcus thailandicus Tanasupawat et al. 2008 is a later subjective synonym
Enterococcus sanguinicola Carvalho et al. 2008, but the name E. sanguinicola is not validly published.
Gram-positive cocci, non-spore-forming, spherical or ovoid, 0.5 – 1 μm in diameter,
arranged in pairs or in chains. Nonmotile.
Colonies are circular, raised or low-convex with entire margins, and non-pigmented.
Weak hemolisis on blood agar. Facultatively anaerobic.  Red colonies appear on
Slanetz–Bartley agar. Grows on kanamycin aesculin azide agar. Grow in 2-6.5% NaCl
medium; weak growth in 8% NaCl. Growth is positive at  15 - 45 ºC and pH 5.0-9.6.
Niacin, calcium pantothenate and folic acid are required for growth. No acidification,
coagulation, reduction or liquefaction in litmus milk.
Enterococcus thailandicus strain was isolated from fermented sausage (‘mum’) in Thailand, and from beavers intestine in Poland.
E. sanguinicola strain was isolated from human blood sample.
  1. Somboon Tanasupawat, Sirapan Sukontasing, and Jung-Sook Lee: Enterococcus thailandicus sp. nov., isolated from fermented
    sausage (‘mum’) in Thailand. Int J Syst Evol Microbiol July 2008 58:1630-1634.
  2. Shewmaker Patricia Lynn; Steigerwalt Arnold G.; Nicholson Ainsley C.; et al.  Reevaluation of the Taxonomic Status of Recently
    Described Species of Enterococcus: Evidence that E. thailandicus Is a Senior Subjective Synonym of "E. sanguinicola" and
    Confirmation of E. caccae as a Species Distinct from E. silesiacus .  JOURNAL OF CLINICAL MICROBIOLOGY  Volume: 49   Issue:
    7   Pages: 2676-2679, Jul 2011.
  3. Maria da Gloria S. Carvalho, Arnold G. Steigerwalt, Roger E. Morey, Patricia Lynn Shewmaker, Enevold Falsen, Richard R.
    Facklam, and Lucia M. Teixeira: Enterococcus sanguinicola sp. nov., Isolated from Human Blood, Comprising the Provisional New
    Species of Enterococcus CDC PNS-E2, and Recognition of Strain Previously Described as Enterococcus CDC PNS-E1 as
    Enterococcus italicus Fortina et al. 2004. J. Clin. Microbiol. 2008.
  4. A. Lauková, A. Kandričáková, J. Imrichová, V. Strompfová, R. Miltko, B. Kowalik and G. Belzecki: Properties of Enterococcus
    thailandicus isolates from beavers. African Journal of Microbiology Research. Vol. 7(28), pp. 3569-3574, 12 July, 2013.
Positive results for hydrolysis of arginine, hydrolysis of aesculin, Voges-Proskauer test, acid production from: D-amygdalin, arbutin,
cellobiose, dulcitol, fructose, D-galactose, beta-gentiobiose, gluconate, D-glucose, glycerol, N-acetylglucosamine, lactose, maltose,
D-mannose, methyl alpha-D-mannoside, D-mannitol, D-ribose, sucrose & salicin.

Negative results for catalase, hydrolysis of gelatin, hydrolysis of starch, hydrolysis of hippurate, reduction of nitrate, production of gas
from glucose, acid production from: adonitol, D- or L-arabinose, D- or L-arabitol, erythritol, D- or L-fucose, 2- or 5-ketogluconate,
methyl alpha-D-glucoside, glycogen, inositol, inulin, D-lyxose, melezitose, melibiose, rhamnose, L-sorbose, starch, trehalose,
turanose, D-tagatose, D-sorbitol, raffinose, xylitol, D- or L-xylose & methyl beta-xyloside.
Enterococcus thailandicus
(c) Costin Stoica
Culture media
Biochemical tests
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