Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Enterococcaceae, Genus Tetragenococcus, Tetragenococcus
osmophilus Juste et al. 2012.
Gram-positive cocci, 0.6-1 µm. Cell division occurs in two planes at right angles to
form tetrads. Non-motile. Non-spore-forming.
Colonies are white, round, convex and smooth, and approximately 0.6 mm in
diameter after incubation at 30 ºC for 5 days on TSA agar. They grow on TSA at 30 ºC
aerobically and in an anaerobic jar. No growth on standard MRS agar at 30 ºC. Growth
is enhanced by the addition of 5% NaCl (w/v). Growth occurs at NaCl concentrations
in the range 0-25 % (w/v) and at 15-30 ºC on TSA agar but not at 4 ºC or above 37 ºC.
Optimal growth temperature is 30 ºC. Optimal pH is 7.0-9.0 Facultatively aerobic.
Isolated from degraded sugar thick juice.
- Juste (A.), Van Trappen (S.), Verreth (C.), Cleenwerck (I.), De Vos (P.), Lievens (B.) and Willems (K.A.): Characterization of
Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides
Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis
subsp. nov. Int. J. Syst. Evol. Microbiol., 2012, 62, 129-137.
Positive results for acid production from: N-acetylglucosamine, arbutin, cellobiose, D-fructose, D-glucose, D-mannose, maltose,
D-mannitol (weak reaction), salicin, sucrose, trehalose & turanose.
Negative results for catalase, esculin hydrolysis, oxidase, acid production from: adonitol, amygdalin, L-arabitol, D- and L-arabinose,
dulcitol, erythritol, D- and L-fucose, glycogen, glycerol, D-galactose, inositol, inulin, lactose, melibiose, melezitose, D-lyxose, raffinose,
L-rhamnose, D-ribose, D-sorbitol L-sorbose, D-tagatose, xylitol, D- and L-xylose & methyl beta-D-xyloside.
Fermentation of gentibiose is variable.
(c) Costin Stoica