Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Enterococcaceae, Genus Enterococcus, Enterococcus haemoperoxidus
Svec et al. 2001 (decompose hydrogen peroxide into oxygen and water on blood-agar media).
Lancefield group D.
Gram-positive, elongated cocci, grouped in pairs, short chains or small groups.
Colonies are circular and smooth with entire margins, 1 mm in diameter after 24 h of
cultivation and up to 3-4 mm after 5 days. A slightly yellowish pigment may be
produced. No hemolysis on sheep-blood agar.
Uniform turbidity produced in BHI broth culture at 37 ºC.
Optimum temperature 37 ºC, grow at 10 ºC but not at 45ºC. Facultatively anaerobic.
Growth enhanced slightly by cultivation in a 5% CO2 atmosphere. Grow in 6.5% NaCl.
Isolated from nonpotable water.
- Pavel Svec and Luc A. Devriese, 2009: Genus I. Enterococcus (ex Thiercelin and Jouhaud 1903) Schleifer and Kilpper-Bälz 1984,
32VP in Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume Three, Vos, P.D.; Garrity, G.; Jones, D.; Krieg, N.R.;
Ludwig, W.; Rainey, F.A.; Schleifer, K.-H.; Whitman, W.B. (Eds.), pp 594-606.
- P Svec, L A Devriese, I Sedlacek, M Baele, M Vancanneyt, F Haesebrouck, J Swings, and J Doskar. Enterococcus haemoperoxidus
sp. nov. and Enterococcus moraviensis sp. nov., isolated from water. Int J Syst Evol Microbiol July 2001 51:1567-74.
Catalase reaction is positive when cultivated on blood agar and negative when is
cultivated on media without blood.
Positive results for acetoin production, hippurate hydrolysis, aesculin hydrolysis, pyrrolidonyl arylamidase, leucine
arylamidase, arginine dihydrolase, acid production from: glycerol, ribose, galactose, D-glucose, D-fructose, D-mannose, methyl
alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, lactose, sucrose, trehalose, melezitose,
beta-gentiobiose & maltotriose.
Negative results for beta-glucuronidase, beta-galactosidase, alkaline phosphatase, urease, acid production from: erythritol, D- or
L-arabinose, D- or L-xylose, adonitol, methyl beta-xyloside, L-sorbose, rhamnose, dulcitol, inositol, sorbitol, methyl
alpha-D-mannoside, melibiose, inulin, D-raffinose, xylitol, D-lyxose, D-tagatose, D- or L-fucose, D- or L-arabitol, gluconate, 2- &
Variable results for acid production from: starch, glycogen, D-turanose & mannitol.
(c) Costin Stoica