Genus Pragia
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Enterobacteriales, Family Enterobacteriaceae, Genus Pragia, Pragia
fontium
Aldova et al. 1988, type species of the genus,.
Gram-negative rods. Motile by peritrichous flagella.
Nonhemolytic colonies on sheep blood agar, 0.5 mm in diameter. Facultatively
anaerobic,  optimum growth temperature 30 ºC. Growth at 4 ºC, but not at 42 ºC.
Grow on media: Nutrient agar or nutrient broth (some strains produce a Shigella-like
odour on nutrient agar), Endo, Mac-Conkey agar, Trypticase Soy Agar ± 5% sheep
blood, TY broth (8 g casein enzymatic hydrolysate type I, 5 g yeast extract powder, 5 g
NaCl per 1,000 ml H
2O).
Isolated from water (17 strains) & feces (1 strain).
Unknown pathogenicity (non-pathogenic).
  1. J. G. Holt et al., 1994. Facultatively Anaerobic Gram-Negative Rods. Subgroup 1. Family Enterobacteriaceae. In: Begey’s Manual of
    Determinative Bacteriology, 9th-edition, Williams & Wilkins, pp 175-189.
  2. Aldova E., Hausner O., Brenner D.J., Kocmoud Z., Schindler J., Potuznikova B. & Petras P.: Pragia fontium gen. nov., sp. nov. of the
    family Enterobacteriaceae, isolated from water. Int. J. Syst. Bacteriol., 1988, 38, 183-189.
  3. Don J. Brenner & J.J. Farmer III, 2004, Family I. Enterobacteriaceae, In: Bergey’s Manual of Systematic Bacteriology, Second edition,
    Vol two, part B, George M. Garrity (Editor-in-Chief), pp. 740-744.
Positive results for H2S production, catalase, nitrates reduction, citrate utilization and
methyl red test.

Negative results for oxidase, indole production, Voges–Proskauer test, phenylalanine deaminase, lysine and ornithine decarboxylases,
arginine dihydrolase, urease, deoxyribonuclease, malonate, ONPG, growth in KCN medium, acid production from L-arabinose,
cellobiose, dulcitol, lactose, maltose, D-mannitol, D-mannose, melibiose, raffinose, L-rhamnose, D-sorbitol, D-sorbose, starch,
sucrose and trehalose.
Acid production from glycerol and myo-inositol may be delayed-positive reaction.
(c) Costin Stoica
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