Taxonomy
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Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium,
Corynebacterium timonense Merhej, Falsen, Raoult and Roux 2009.
Gram-positive typically club-shaped rods, 0.6-2.1 x 0.4-0.6 µm, occuring as single
cells, in pairs or in small clusters. Non-spore-forming. Non-motile. Tuberculostearic
acid are not present.
Colonies on sheep blood agar are circular, greyish, glistening and 0.5– 1 mm in
diameter after 48 h at 37 ºC. Non-haemolytic. Grows in TSB. Grows at  25-50 ºC; 37 ºC
optimum. Aerobic and anaerobic growth.
Isolated from the blood of a patient with endocarditis. Sensitive to amoxicillin, doxycycline, rifampicin, vancomycin, trimethoprim–
sulfamethoxazole, gentamicin, ofloxacin and clindamycin. Resistant to erythromycin.
The type strain was isolated from the blood of a patient with endocarditis and persistent fever.
  1. Merhej V., Falsen E., Raoult D. and Roux V., 2009. Corynebacterium timonense sp. nov. and Corynebacterium massiliense sp.
    nov., isolated from human blood and human articular hip fluid. IJSEM 59, 1953-1959.
  2. Bernard K.A. and Funke G., 2012. Genus I. Corynebacterium Lehmann and Neumann 1896, emend. Bernard, Wiebe, Burdz,
    Reimer, Ng, Singh, Schindle and Pacheco 2010, in: Goodfellow M., Kämpfer P., Busse H.J., Trujillo M.E., Suzuki K.I., Ludwig W.
    and Whitman W.B. (Editors) Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume Five, The Actinobacteria, Part A,
    pp 245-289.
Fermentative metabolism. Most results are based on API CORYNE and API ZYM tests.

Positive results for acid phosphatase, alkaline phosphatase, catalase, esterase (C4),
aesculin hydrolysis (weak), esterase lipase (C8), lipase, leucine arylamidase, pyrazinamidase, valine arylamidase, acid production
from glucose & ribose.

Negative results for cystine arylamidase, alpha-chymotrypsin, gelatin hydrolysis, beta-glucuronidase, beta-galactosidase, alpha- and
beta-glucosidase, N-acetyl-beta-glucosaminidase, nitrate reduction, oxidase, pyrrolidonyl arylamidase, trypsin, urease, acid production
from: glycogen, lactose, maltose, mannitol,  sucrose & xylose.
Corynebacterium timonense
(c) Costin Stoica
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