Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium sputi Yassin and Siering 2008.
Species description is based on a single isolate.
Gram-positive coryneform rods. Non-motile. Non-spore-forming. Tuberculostearic
acid is present. Mycolic acids are produced.
Colonies are cream-yellow, convex, dry and approximately 0.2–2 mm in diameter after
48 h incubation at 37 ºC on sheep-blood agar in aerobic conditions. Tween 80
encourages the growth. Non-haemolytic. Facultatively anaerobic.
Isolated from sputum.
Isolated from the sputum of a patient with pneumonia.
- Yassin A.F. and Siering C., 2008. Corynebacterium sputi sp. nov., isolated from the sputum of a patient with pneumonia. IJSEM
Description is based on API CORYNE (2011104), API 20 Strep and API ZYM tests.
Positive results for catalase, ester lipase (C8), lipase C14, lipophilism, leucine arylamidase, pyrazinamidase, urease and acid
production from glucose.
Negative results for acid phosphatase, alkaline phosphatase, aesculin hydrolysis, cysteine arylamidase, esterase (C4), gelatin
hydrolysis, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase,
hippurate hydrolysis, nitrate reduction, oxidase, pyrrolidonyl arylamidase, trypsin, chymotrypsin, valine arylamidase, Voges-Proskauer
reaction, acid production from: L-arabinose, glycogen, inulin, lactose, maltose, mannitol, raffinose, ribose, starch, sorbitol, sucrose,
trehalose & xylose.
(c) Costin Stoica