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Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium,
Corynebacterium sphenisci Goyache, Ballesteros, Vela, Collins, Briones, Hutson,
Potti, Garcia-Borboroglu, Dominguez and Fernandez-Garayzabal 2003.
Gram-positive coryneform rods. Non-motile. Non-spore-forming. Tuberculostearic
acid is not present. Mycolic acids are produced in small amounts.
Colonies are whitish, low-convex, dry and rough and approximately 1-2 mm in
diameter after 48 h incubation at 37 ºC on sheep-blood agar. CAMP-negative.
Non-haemolytic. Facultatively anaerobic. Can grow at 20 ºC.
Isolated from the cloacae of apparently healthy wild magellanic penguins. Susceptible to O/129 vibriostatic agent.
Undetermined.
  1. Goyache J., Ballesteros C., Vela A.I., Collins M.D., Briones V., Hutson R.A., Potti J., Garcia-Borboroglu P., Dominguez L. and
    Fernandez-Garayzabal J.F., 2003. Corynebacterium sphenisci sp. nov., isolated from wild penguins. IJSEM 53, 1009-1012.
Description is based on API CORYNE (3000124), API 50CH and API ZYM tests.
Fermentative metabolism.

Positive results for acid phosphatase, catalase, chymotrypsin, cysteine arylamidase, esterase (C4), ester lipase (C8), leucine
arylamidase, lipase, nitrate reduction, pyrazinamidase, valine arylamidase, acid production from: fructose, galactose, glucose (and at
42 ºC), maltose, mannose & trehalose.

Negative results for alkaline phosphatase, aesculin hydrolysis, alpha- and beta-galactosidase, alpha- and beta-glucosidase,
beta-glucuronidase, N-acetyl-beta-glucosaminidase, gelatin hydrolysis, lipophilic reaction, oxidase, pyrrolidonyl arylamidase, trypsin,
urease, acid production from: adonitol, amygdalin, L-arabinose, arabitol, arbutin, cellobiose, dulcitol, erythritol,L- and D-fucose,
glycerol, glycogen, gluconate, 2- and 5-ketogluconate, N-acetyl-beta-glucosamine, gentiobiose, inositol, inulin, lactose, lyxose,
mannitol, melezitose, melibiose, raffinose, rhamnose, ribose, salicin, sorbitol, L-sorbose, starch, sucrose, turanose, xylitol & xylose.
Corynebacterium sphenisci
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