Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium simulans Wattiau, Janssens and Wauters 2000.
Gram-positive, diphtheroid rods. Nonspore-forming. Nonmotile.
Colonies are greyish-white, glistening, and 1-2 mm in diameter after 48 h of
incubation at 37 ºC on 5% sheep blood agar. CAMP test is negative. Facultatively
anaerobic. Growth at 20 ºC dw/3 days.
Isolated from skin-related specimens (a foot abscess, a biopsy of an axillar lymph node and a boil, respectively).
Susceptible to penicillin, cephalotin, ciprofloxacin, gentamicin, rifampicin, erythromycin, ampicillin and amoxicillin+clavulanic acid.
Isolated from skin-related specimens (a foot abscess, a biopsy of an axillar lymph node and a boil, respectively) but disease
association was not proved.
- Wattiau P., Janssens M. and Wauters G. 2000. Corynebacterium simulans sp. nov., a nonlipophilic, fermentative
Corynebacterium. IJSEM 50, 347-353.
- Funke G ., 2006. Corynebacteria and rare coryneforms. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition,
Edited by Borriello S.P., Murray P.R. and Funke G., Edward Arnold (Publishers) Ltd., Bacteriology, volume 2.
Most results are based on API CORYNE, API 50CH and API ZYM tests.
Positive results for alkaline phosphatase, catalase, esterase (C4), esterase lipase
(C8), nitrate reduction, nitrite reduction, acid production from: fructose, glucose,
mannose & sucrose.
Negative results for esculin hydrolysis, gelatin hydrolysis, beta-galactosidase, beta-glucuronidase, alpha-glucosidase, lipophilism,
urease, acid production from: adonitol, amygdalin, arabinose, arabitol, arbutin, cellobiose, dulcitol, erythritol, esculin, ethylene glycol,
fucose, gluconate, 2-ketogluconate, beta-gentiobiose, glycerol, glycogen, inositol, inulin, 5-ketogluconate, lactose, lyxose,maltose,
mannitol, melezitose, melibiose, raffinose, rhamnose, salicin,sorbitol, sorbose, starch, tagatose, turanose, xylitol & xylose.
Variable results for pyrazinamidase, acid production from: N-acetylglucosamine, galactose & ribose.
(c) Costin Stoica