Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium nuruki Shin, Jung, Kim, Roh, Nam and Bae 2011.
Species description is based on a single isolate.
Gram-positive irregular rod-shaped cells. Non-motile. Mycolic acids are present.
Colonies on PY-BHI (peptone-yeast-brain-heart infusion) agar medium are smooth,
opaque, convex, 0.5–1.0 mm in diameter, slightly viscous and cream-coloured after
48 h at 30 ºC. Grows on nutrient agar (NA; Difco), tryptic soy agar (TSA; Bacto),
Columbia agar (BBL) supplemented with 5 % horse blood and Corynebacterium
agar (DSM medium no. 53) containing 1 % tryptone, 0.5 % yeast extract, 0.5 %
glucose and 0.5 % NaCl (pH 7.2). Strictly aerobic. Grows at/in: 10-45 ºC (37 ºC
optimum). Grows in 0-10% NaCl (1 % NaCl optimum). Grows at pH 6.0-9.0 (pH 8.0
Isolated from nuruk, an alcohol fermentation starter of traditional Korean wine.
- Shin N.R., Jung M.J., Kim M.S., Roh S.W., Nam Y.D. and Bae J.W., 2011. Corynebacterium nuruki sp. nov., isolated from an
alcohol fermentation starter. IJSEM 61, 2430-2434.
Description is based on API CORYNE, API 50CH and API 50CHB results.
Positive results for catalase, alkaline phosphatase, oxidase, pyrazinamidase, acid production from: fructose, galactose, glucose,
5-ketogluconate, mannose, methyl alpha-D-glucoside & ribose.
Negative results for beta-galactosidase, beta-glucuronidase, alpha and beta-glucosidase, N-acetyl-beta-glucosaminidase, nitrate
reduction, pyrrolidonyl arylamidase, urease, acid production from: arabitol, aesculin, arbutin, cellobiose, gluconate, glycerol, mannitol
Assimilates: D-ribose, D-lxylose, D-adonitol, D-galactose, D-glucose, D-fructose, D-mannose, L-sorbose, L-rhamnose, dulcitol,
inositol, D-mannitol, D-sorbitol, methyl a-D-glucoside, N-acetylglucosamine, amygdalin, aesculin, lactose, inulin, melezitose,
gentiobiose, turanose, D-lyxose, gluconate & 5-ketogluconate (API 50CH).
(c) Costin Stoica