Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium mucifaciens Funke, Lawson and Collins 1997.
Gram-positive diphtheroids. Nonmotile. Nonspore-forming.
Colonies are glistening, convex and 1-1.5 mm in diameter after 24 h of incubation at
37 ºC in a 5% CO2-enriched atmosphere on sheep blood Columbia agar colonies are
very distinct because they are slightly to overtly yellow and very mucoid (correlate,
probably, with extracellular polysaccharides). CAMP reaction is negative.
Mainly isolated from human blood culture and other sterile body fluids. Sensitive to beta-lactams and aminoglycosides.
- Funke G., Efstratiou A., Kuklinska D., Hutson R.A., De Zoysa A., Engler K.H. and Collins M.D., 1997. Corynebacterium mucifaciens
sp. nov.,an unusual species from human clinical material. IJSB. Vol. 47, No.4, 952-957.
- Funke G ., 2006. Corynebacteria and rare coryneforms. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition,
Edited by Borriello S.P., Murray P.R. and Funke G., Edward Arnold (Publishers) Ltd., Bacteriology, volume 2.
Description of most biochemical tests is based on API CORYNE, 50CH and ZYM.
Positive results for acid phosphatase, alkaline phosphatase, catalase, cystine
arylamidase, esterase (C4), esterase lipase (C8), pyrazinamidase, acid production
from: fructose, glucose, glycerol & mannose.
Negative results for chymotrypsin activity, esculin hydrolysis, lipophilism, nitrate reduction, urease, acid production from:
N-acetylglucosamine, adonitol, amygdalin, D- and L-arabinose, arabitol, arbutin, cellobiose, dulcitol, erythritol, L- and D-fucose,
galactose, beta-gentiobiose, gluconate, 2-ketogluconate, glycogen, inositol, inulin, lactose, lyxose, maltose, mannitol,
melezitose,melibiose, raffinose, rhamnose, salicin, sorbitol, sorbose, tagatose, trehalose, turanose, xylitol & xylose.
Variable results for acid production from: ribose & sucrose.
(c) Costin Stoica