Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium massiliense Merhej, Falsen, Raoult and Roux 2009.
Gram-positive typically club-shaped rods, 0.2-1.8 x 0.3-0.7 µm, occuring as single
cells, in pairs or in small clusters. Non-spore-forming. Non-motile. Tuberculostearic
acids are present.
Colonies on sheep blood agar are circular, greyish, glistening and 0.5– 1 mm in
diameter after 48 h at 37 ºC. Non-haemolytic. Grows in TSB. Grows at 30-44 ºC; 37 ºC
optimum. Aerobic; anaerobic growth is weak.
Isolated from human hip joint fluid. Sensitive to amoxicillin, doxycycline, rifampicin, vancomycin, trimethoprim–sulfamethoxazole,
gentamicin, ofloxacin, erythromycin and clindamycin.
The type strain was isolated from human hip joint fluid (an 84-year-old man who had previously undergone arthroplasty of his left hip
presented with left knee pain and intermittent fever).
- Merhej V., Falsen E., Raoult D. and Roux V., 2009. Corynebacterium timonense sp. nov. and Corynebacterium massiliense sp.
nov., isolated from human blood and human articular hip fluid. IJSEM 59, 1953-1959.
- Bernard K.A. and Funke G., 2012. Genus I. Corynebacterium Lehmann and Neumann 1896, emend. Bernard, Wiebe, Burdz,
Reimer, Ng, Singh, Schindle and Pacheco 2010, in: Goodfellow M., Kämpfer P., Busse H.J., Trujillo M.E., Suzuki K.I., Ludwig W.
and Whitman W.B. (Editors) Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume Five, The Actinobacteria, Part A,
Oxidative metabolism. Most results are based on API CORYNE and API ZYM tests.
Positive results for alkaline phosphatase (weak), catalase, esterase (C4) (weak),
esterase lipase (C8) (weak), leucine arylamidase & pyrazinamidase (weak).
Negative results for acid phosphatase, aesculin hydrolysis, cystine arylamidase, alpha-chymotrypsin, gelatin hydrolysis, beta-
glucuronidase, beta-galactosidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, lipase, nitrate reduction, oxidase,
pyrrolidonyl arylamidase, trypsin, urease, valine arylamidase, acid production from: glucose, glycogen, lactose, maltose, mannitol,
ribose, sucrose & xylose.
(c) Costin Stoica