Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium macginleyi Riegel, Ruimy, De Briel, Prevost, Jehl, Christen and
Historical synonym: CDC coryneform group G-1 (genomospecies III).
Gram-positive, pleomorphic rods with occasionally metachromatic granules,
arranged in palisades or V-shaped forms. Nonmotile. Nonspore-forming.
No growth in BHI broth but is visible growth in BHI broth supplemented with 0.01-1%
Tween 80 after 24 h at 37 ºC. Better growth is observed on SBA (sheep blood agar)
plates supplemented with 0.1 % Tween 80 rather with 1% and some strains exhibit a
rose pigment: colonies are large, 2-4 mm in diameter and Tween esterase activity is
noted. The colonies are very small (> 1 mm in diameter) on SBA without Tween 80.
Facultatively anaerobic. Beta-hemolysis and CAMP reaction are negative.
Isolated almost exclusively from eye specimens. Bacterium is susceptible to a broad spectrum of antimicrobial agents.
The type strain was isolated from the eye of a patient suffering from a corneal ulcer.
- Funke G., von Graevenitz A., Clarridge III J.E., and Bernard K.A., 1997. Clinical Microbiology of Coryneform Bacteria. Clinical
Microbiology Rewiews Vol. 10, No. 1, p. 125-159.
- Funke G ., 2006.Corynebacteria and rare coryneforms. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition,
Edited by Borriello S.P., Murray P.R. and Funke G.,Edward Arnold (Publishers) Ltd., Bacteriology, volume 2.
- Riegel P., Ruimy R., De Briel D., Prevost G., Jehl F., Christen R., and Monteil H., 1995. Genomic Diversity and Phylogenetic
Relationships among Lipid-Requiring Diphtheroids from Humans and Characterization of Corynebacterium macginleyi sp. nov.
IJSB Vol. 45, No. 1, p. 128-133.
Fermentative metabolism. Not acid fast. Tests description is based on API Coryne kit.
Positive results for alkaline phosphatase, catalase, DNA degradation, lipophilism, nitrate reduction, acid production from: glucose,
ribose & sucrose.
Negative results for arginine degradation, esculin hydrolysis, gelatin hydrolysis, oxidase, pyrazinamidase, starch hydrolysis, tyrosine
degradation, urease, acid production from: glycogen, lactose, maltose, trehalose & xylose.
Variable results for hippurate hydrolysis & acid production from mannitol.
(c) Costin Stoica