Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium lipophiloflavum Funke, Hutson, Hilleringmann, Heizmann and
Description is based on a single isolate.
Gram-positive rods may appear coccoidal after prolonged incubation, 0.5-1.0 µm in
diameter, arranged in V forms or palisades. Nonsporing. Nonmotile.
It is fastidious and slow-growing. Good growth on cystine lactose electrolyte-deficient
agar. Growth is stimulated by Tween 80.
Colonies are convex, yellow, smooth, pinpoint after 48 h of incubation at 25-42 ºC on
sheep blood agar under CO2; they grow in 7% CO2 as tiny, strong yellow colonies on
chocolate agar and cystine lactose electrolyte-deficient agar.
CAMP reaction is negative. Hemolysis is negative. Grow at: 25-42 ºC. Strictly aerobes.
Isolated from human.
Isolated from a patient with bacterial vaginosis.
- Funke G ., 2006. Corynebacteria and rare coryneforms. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition,
Edited by Borriello S.P., Murray P.R. and Funke G., Edward Arnold (Publishers) Ltd., Bacteriology, volume 2.
- Guido Funke, Roger A Hutson, Markus Hilleringmann, Wolfgang R Heizmann, Matthew D Collins. Corynebacterium
lipophiloflavum sp. nov. isolated from a patient with bacterial vaginosis. FEMS Microbiology Letters, 219-224 First published
online: 1 May 1997.
Oxidative metabolism. Non-acid-fast. Mycolic acids are present. Most biochemical characters were tested on API Coryne kits. The
commercial API Coryne identification system gave the numerical code 2101004 which corresponded with the identification of the
isolates as C. urealyticum.
Positive results for alkaline phosphatase, acid phosphatase, catalase, lipophilism, esterase, esterase lipase, lipase, leucine
arylamidase pyrazinamidase, Tween 80 hydrolysis & urease.
Negative results for esculin hydrolysis, gelatin hydrolysis, nitrate reduction, cystine arylamidase, valine arylamidase, trypsin,
chymotrypsin, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, alpha-mannosidase,
alpha-fucosidase, acid production from: arabinose, glucose, glycogen, inulin, lactose, maltose, mannitol, raffinose, ribose, sorbitol,
starch, sucrose, trehalose & xylose.
(c) Costin Stoica