Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium lactis Wiertz, Schulz, Müller, Kämpfer and Lipski 2013.
Gram-positive pleomorphic short rods, 1.0-1.6 x 0.6-0.8 µm. Non-spore-forming.
Non-motile. Tuberculostearic acid is absent. Mycolic acids are not present.
Colonies on TSA after 3 days of incubation under aerobic conditions at 30 ºC are
about 2 mm in diameter, white, white, with uneven edge and dry. On Columbia blood
agar colonies are about 4 mm in diameter, white, with uneven edge and dry.
Non-haemolytic. Can grow at 42 ºC, but not at 20 ºC.
Isolated from raw cow’s milk produced in Germany.
- Wiertz R., Schulz S.C., Muller U., Kampfer P. and Lipski A., 2013. Corynebacterium frankenforstense sp. nov. and
Corynebacterium lactis sp. nov., isolated from raw cow milk. IJSEM 63, 4495-4501.
Most biochemical reactions were determined by API Coryne, API 50CH, and API ZYM.
Positive results for catalase, pyrazinamidase, acid production from: fructose, glucose
at 42 ºC and 37 ºC, mannose & ribose.
Negative results for beta-galactosidase, gelatin hydrolysis, alpha- or beta-glucosidase, N-acetyl-beta-glucosaminidase, lipase (C14),
nitrate reduction, oxidase, starch hydrolysis, urease, acid production from: adonitol, amygdalin, L- or D-arabinose, D-arabitol, arbutin,
N-acetyl-glucosamine, cellobiose, dulcitol, erythritol, fucose, galactose, gentiobiose, gluconate, 2 or 5-ketogluconate, glycogen,
inositol, inulin, lactose, lyxose, maltose, mannitol, melezitose, melibiose, raffinose, rhamnose, sorbitol, sorbose, starch, tagatose,
turanose, xylitol & xylose.
Variable results for alkaline phosphatase, acid phosphatase, esterase lipase, beta-glucuronidase, acid production from: galactose,
sucrose & trehalose.
(c) Costin Stoica