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Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium,
Corynebacterium humireducens  Wu, Zhuang, Zhou, Li and He 2011.
Gram-positive straight to curved rods, 1.0-2.0/0.5-0.7 µm, occurred singly or in pairs.
Non-spore-forming. Non-motile.
Colonies on LB agar are round, 0.5–1.0 mm in diameter, yellowish, dry and rough,
with a distinct wrinkled morphology after 24 hours. Optimum growth at 37 ºC (25-40
ºC limits), pH 9.0 (7.0-11.0 limits) and with 10% NaCl in aerobiosis. Facultatively
anaerobic.
Isolated from a microbial fuel cell fed with artificial wastewater and operated continuously at pH 10.0.
Undetermined.
  1. Wu C.Y., Zhuang L., Zhou S.G., Li F.B. and He J., 2011. Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-
    reducing bacterium isolated from a microbial fuel cell. IJSEM 61, 882-887.
Most biochemical reactions were determined by API Coryne.

Positive results for alkaline phosphatase, catalase, esterase, esterase lipase, lipase, pyrazinamidase, acid production from: glucose
& ribose.

Negative results for aesculin hydrolysis, gelatin hydrolysis, beta-galactosidase, alpha-glucosidase, beta-glucuronidase, methyl red,  
N-acetyl-beta-glucosaminidase, nitrate reduction, oxidase, starch hydrolysis, tyrosine hydrolysis, urease, acid production from:
arabinose, fructose, galactose, lactose, maltose, mannose, mannitol, raffinose, rhamnose, salicin, sucrose, trehalose & xylose.
Corynebacterium humireducens
(c) Costin Stoica
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