Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium freneyi Renaud, Aubel, Riegel, Meugnier and Bollet 2001 emend.
Funke and Frodl 2008.
Gram-positive typically club-shaped rods. Nonmotile. Nonspore-forming.
On TSA plates supplemented with 5% sheep blood at 37 ºC in a 10% CO2
atmosphere the colonies are whitish-grayish or yellowish, dry, rough, with irregular
edges, up to 2 mm in diameter after 48 h incubation. Can grow at 20 ºC. CAMP test is
Isolated from skin-related material (from varicose ulcer, a subcutaneous abscess fistula, pus from a toe), vaginal swab, uterine cervix,
outer ear canal, duodenal biopsy, urine.
Sensitive to doxycycline, rifampin, tobramycin, gentamicin, tetracycline, vancomycin, O/129 and oxacillin. Resistant to fosfomycin.
- Renaud F.N.R., Aubel D., Riegel P., Meugnier H. and Bollet C., 2001. Corynebacterium freneyi sp. nov., α-glucosidase-positive
strains related to Corynebacterium xerosis. IJSEM 51, 1723-1728.
- Funke G., 2006. Corynebacteria and rare coryneforms. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition,
Edited by Borriello S.P., Murray P.R. and Funke G., Edward Arnold (Publishers) Ltd., Bacteriology, volume 2.
- Funke G. and Frodl R., 2008. Comprehensive Study of Corynebacterium freneyi Strains and Extended and Emended Description
of Corynebacterium freneyi Renaud, Aubel, Riegel, Meugnier, and Bollet 2001. J. Clin. Microb. Vol. 46, No. 2 , p 638-643.
Description of most biochemical tests is based on API CORYNE kits.
Positive results for alkaline phosphatase, catalase, esterase (C4), esterase lipase (C8), alpha-glucosidase, pyrazinamidase, acid
production from: fructose, glucose (at 42 ºC), 5-ketogluconate, maltose, mannose, ribose (weak reaction), sucrose, tagatose (weak
reaction), trehalose & turanose.
Negative results for esculin hydrolysis, gelatine hydrolysis, beta-galactosidase, beta-glucuronidase, lipophilism, urease, acid
production from: arabinose, glycerol, 2-ketogluconate, mannitol & xylose.
Variable results for nitrate reduction, acid production from: glycogen & lactose.
(c) Costin Stoica