Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium freiburgense Funke, Frodl, Bernard and Englert 2009.
Description is based on a single isolate.
Gram-positive, typically club-shaped rods that occur as single cells, in pairs or in
small clusters. Non-spore-forming. Non-motile. Mycolic acids are not present.
Cultured on Columbia blood agar base supplemented with 5% defibrinated sheep
blood at 37 ºC in air plus 5% CO2; colonies are beige–whitish, dryish, convoluted with
irregular edges, strongly adherent to agar and up to 1– 2 mm in diameter after 48 h
incubation. In 5-day-old colonies, a ‘spoke-wheel’ macroscopic morphology may be
observed. Weak anaerobic growth. CAMP test is negative.
Isolated from a patient’s wound obtained from a dog bite. Susceptible to cefotaxime, ciprofloxacin, doxycycline, erythromycin,
gentamicin, linezolid, meropenem, penicillin, rifampicin, vancomycin and vibriocidal compound O/129.
- Funke G., Frodl R., Bernard K.A. and Englert R., 2009. Corynebacterium freiburgense sp. nov., isolated from a wound obtained
from a dog bite. IJSEM 59, 2054-2057.
Some biochemical reactions were determined by API Coryne (1440365).
Positive results for acid phosphatase, catalase, esculin hydrolysis (delayed: after 72 h only), ester lipase (C8), esterase (C4),
beta-galactosidase, beta-glucosidase, nitrate reduction, acid production from: fructose, galactose, glucose, 5-ketogluconate, lactose,
maltose, mannose, ribose, sucrose & tagatose.
Negative results for alkaline phosphatase, alpha-galactosidase, alpha-glucosidase, beta-glucuronidase, gelatin hydrolysis,
N-acetyl-beta-glucosaminidase, lipophilic reaction, pyrazinamidase, urease, acid production from: adonitol, amygdalin, arabinose,
arabitol, arbutin, cellobiose, dulcitol, erythritol, fucose, gentiobiose, gluconate, glycerol, glycogen,, methyl alpha-D-glucoside,
N-acetyl-beta-glucosamine, mannitol, inositol, inulin, 2-ketogluconate, lyxose, melezitose, melibiose, methyl alpha-D-mannoside,
raffinose, rhamnose, salicin, sorbitol, sorbose, starch, trehalose, turanose, xylitol, xylose & methyl beta-xyloside.
(c) Costin Stoica