Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium, Corynebacterium doosanense Lee, Cho, Jung, Nguyen, Jung, Park, Le and Kim 2009.
Description is based on a single isolate.
Gram-positive irregular and club-shaped rods, a typical coryneform morphology,
0.8-1.0 x 1.0-1.2 µm. Mycolic acids are present. Nonspore-forming. Nonmotile.
Isolated on glucose-yeast extract agar supplemented with cycloheximide and nalidixic
acid after 3 days of aerobically incubation at 30 ºC; opaque, yellow, low-convex
bacterial colony with a diameter of 1–2 mm was subcultured on sheep blood agar
plates. Haemolysis and CAMP test negative.
Isolated from activated sludge taken from a wastewater treatment plant in Yeongdeuk-gun, Republic of Korea.
- Lee H.J., Cho S.L., Jung M.Y., Nguyen T.H.V., Jung Y.C., Park H.K., Le V.P. and Kim W., 2009. Corynebacterium doosanense sp.
nov., isolated from activated sludge. IJSEM 59, 2734-2737.
Most biochemical reactions were determined by API Coryne, API ZYM and API Strep.
Positive results for catalase, esterase lipase (C8), hippuric acid hydrolysis, oxidase, pyrazinamidase, acid production from glucose &
Negative results for N-acetyl-beta-glucosaminidase, acid phosphatase, alkaline phosphatase, esculin hydrolysis, esterase (C4),
gelatin hydrolysis, beta-galactosidase, alpha-glucosidase, beta-glucuronidase, lipase (C14), nitrate reduction, trypsin, urease, acid
production from glycogen, lactose, mannitol, maltose, ribose & xylose.
(c) Costin Stoica