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Phylum Actinobacteria, Class Actinobacteria , Subclass Actibacteridae, Order Actynomycetales, Suborder Corynebacterinae, Family
Corynebacteriaceae, Genus Corynebacterium,
Corynebacterium doosanense Lee, Cho, Jung, Nguyen, Jung, Park, Le and Kim 2009.

Description is based on a single isolate.
Gram-positive irregular and club-shaped rods, a  typical coryneform morphology,
0.8-1.0 x 1.0-1.2 µm. Mycolic acids are present. Nonspore-forming. Nonmotile.
Isolated on glucose-yeast extract agar supplemented with cycloheximide and nalidixic
acid after 3 days of aerobically incubation at 30 ºC; opaque, yellow, low-convex
bacterial colony with a diameter of 1–2 mm was subcultured on sheep blood agar
plates. Haemolysis and CAMP test negative.
Isolated from activated sludge taken from a wastewater treatment plant in Yeongdeuk-gun, Republic of Korea.
Undetermined.
  1. Lee H.J., Cho S.L., Jung M.Y., Nguyen T.H.V., Jung Y.C., Park H.K., Le V.P. and Kim W., 2009. Corynebacterium doosanense sp.
    nov., isolated from activated sludge. IJSEM 59, 2734-2737.
Most biochemical reactions were determined by API Coryne, API ZYM and API Strep.

Positive results for catalase, esterase lipase (C8), hippuric acid hydrolysis, oxidase, pyrazinamidase, acid production from glucose &
sucrose.

Negative results for N-acetyl-beta-glucosaminidase, acid phosphatase, alkaline phosphatase, esculin hydrolysis, esterase (C4),
gelatin hydrolysis, beta-galactosidase, alpha-glucosidase, beta-glucuronidase, lipase (C14), nitrate reduction, trypsin, urease, acid
production from glycogen, lactose, mannitol, maltose, ribose & xylose.
Corynebacterium doosanense
(c) Costin Stoica
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