Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Micrococcinae, Family
Microbacteriaceae, Genus Rathayibacter, Rathayibacter caricis Dorofeeva, Evtushenko, Krausova, Karpov, Subbotin and Tiedje 2002.
Gram-positive, irregular rods, 0.5-0.8 / 2.0-2.5 µm, and occurred singly or in pairs with
diphtheroid arrangements; in 5-7-day cultures, the rods were replaced by coccoid or
coccobacillus forms, 0.6-1.0 µm in diameter. Non-motile. Non-spore-forming.
Colonies on Corynebacterium Agar are yellow, butyrous, opaque, convex, 1.0 mm in
diameter, visible after 2-3 days incubation. Obligately aerobic.
Grows at 24-26 ºC, optimum. No growth at 37 ºC. Weak growth in 5% NaCl media. No
growth in 0.03% tellurium acetate.
Isolated from phyllosphere of Carex sp. (Belgorod region, Russia). Probably is associated with a nematode of the subfamily Anguininae.
Resistant to ampicillin, gentamicin and rubomicin. Susceptible to kerbonicillin, levomicetin, metacycline, rifampicin and streptomycin.
- Zgurskaya H.I., Evtushenko L.I., Akimov V.N. and Kalakoutskii L.V. 1993. Rathayibacter gen. nov., Including the Species
Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and Six Strains from Annual
Grasses. Int. J. Syst. Bacteriol. Vol. 43, No.1, 143-149.
- Dorofeeva L.V., Evtushenko L.I., Krausova V.I., Karpov A.V., Subbotin S.A. and Tiedje J.M., 2002. Rathayibacter caricis sp. nov. and
Rathayibacter festucae sp. nov., isolated from the phyllosphere of Carex sp. and the leaf gall induced by the nematode Anguina
graminis on Festuca rubra L., respectively. Int. J. Syst. Evol. Microbiol. 52, 1917-1923.
Positive results for catalase, H2S production (weak), methyl red reduction, Tween 40 hydrolysis & Voges-Proskauer reaction.
Negative results for aesculin hydrolysis, casein hydrolysis, citrate utilisation, hippurate hydrolysis, nitrate reduction, starch hydrolysis,
Tween 60, 80 hydrolysis, urease, acid production from: dulcitol, inositol, lactose, mannitol, melibiose, rhamnose & sorbitol.
Variable results for oxidase or weak positive, depending on growth phase.
(c) Costin Stoica