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Phylum Actinobacteria, Class Actinobacteria, Order Micrococcales, Family Brevibacteriaceae, Genus Brevibacterium, Brevibacterium
sanguinis
Wauters, Haase, Avesani, Charlier, Janssens, Van Broeck and Delmee 2004.
Gram-positive coryneform rods. Nonmotile.
Colonies are opaque, smooth or somewhat sticky, greyish-white, up to 2 mm in
diameter after 48 h at 37 ºC aerobically incubation on Sheep
Blood Agar.
Grows at 20
-37 ºC. Can grow in 10% NaCl media.
Isolated from human clinical samples, sterile body sites (blood and dialysate fluid).
Very susceptible to thallium acetate. Resistant to penicillin. Susceptible to cephalothin, gentamicin, erythromycin, ciprofloxacin  and
vancomycin
.
No pathological role was proved; clinical significance remains to be proved, especially for HIV patients.
  1. Wauters G., Haase G., Avesani V., Charlier J., Janssens M., Van Broeck J., and Delmee M., 2004. Identification of a novel
    Brevibacterium species isolated from humans and description of Brevibacterium sanguinis sp. nov. J Clin Microbiol Vol 42, No. 6,
    2829-2832.
Description is based on API ZYM, API 50CH and API Biotype 100 with AUX medium,
API 32 GN, API CORYNE (code similar with Brevibacterium casei: 6112004).

B. sanguinis is very similar to B. casei when tested by conventional methods or commercial identification sets but susceptibility to
thallium acetate may help to differentiate the two species in the routine laboratory.


Positive results for
acid phosphatase, alkaline phosphatase, catalase, casein hydrolysis, esterase (C4), esterase lipase (C8),  
gelatin hydrolysis, alpha-glucosidase, N-acetyl-beta-glucosaminidase (weak reaction), leucine arylamidase, phosphoamidase,
pyrazinamidase, tyrosine hydrolysis, xanthine hydrolysis, acid production from phenyl acetate.

Negative results for
aesculin hydrolysis, beta-galactosidase, beta-glucuronidase, urease, acid production from: D-glucose, glycogen,
lactose, maltose, mannitol, mannose,  raffinose, ribose, sucrose & xylose. No other carbohydrates are acidified.

Variable results for pyrrolidonyl arylamidase. Nitrate reduction is weak or negative.
Brevibacterium sanguinis
(c) Costin Stoica
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