Phylum Actinobacteria, Class Actinobacteria, Order Micrococcales, Family Brevibacteriaceae, Genus Brevibacterium, Brevibacterium
massiliense Roux and Raoult 2009.
Gram-positive straight, irregular, short rods, 0.3-0.5 / 0.4-1.4 µm. Mycolic acids are
absent. Nonmotile. Spores are not produced.
Colonies are shiny, beige, smooth, 0.2 mm in diameter after 1 day in 5% CO2 on
sheep blood agar. Aerobic. Grows at 25-45 ºC (30-37 ºC optimum). Grows in 0-10%
Isolated from a human ankle discharge sample in Marseille.
- Roux V. and Raoult D., 2009. Brevibacterium massiliense sp. nov., isolated from a human ankle discharge. Int J Syst Evol
Microbiol 59, 1960-1964.
Most characters are based on API ZYM, API Coryne and API Rapid ID 32A strips results.
Positive results for catalase, esterase (C4), esterase lipase (C8) (weak reaction),
leucine arylamidase, phosphoamidase & pyrazinamidase (weak reaction).
Negative results for acid phosphatase, alkaline phosphatase, arginine dihydrolase, cystine arylamidase, alpha-fucosidase, gelatin
hydrolysis, alpha- and beta-galactosidase, alpha- and beta-glucosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase, indole
production, lipase (C14), alpha-mannosidase, nitrate reduction, oxidase, pyrrolidonyl arylamidase, trypsin, alpha-chymotrypsin,
urease, valine arylamidase, acid production from: D-glucose, glycogen, lactose, maltose, mannitol, mannose, raffinose, ribose,
sucrose & xylose.
(c) Costin Stoica