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Phylum Actinobacteria, Class Actinobacteria, Order Micrococcales, Family Brevibacteriaceae, Genus Brevibacterium, Brevibacterium
marinum
Lee 2008.
Gram-positive rods, 0.6-0.9 / 1.5-2.4 µm; pairs, chains, V-form arrangement. Mycolic
acids are absent. Non-motile. Non-spore-forming.
Colonies are convex, translucent, smooth, with entire margins; in the dark colonies
are white and in the light are bright yellow. Grows well in/on TSB, TSA, nutrient agar,
yeast extract-malt agar (ISP 2 medium), with and without 60% seawater. Grows at
10-30 ºC (30 ºC optimum), but not at 4 or 37 ºC. Grows at 5.1-12.1 pH and in 0-10%
NaCl medium.
Isolated from seawater from Hwasun Beach in Jeju, Republic of Korea.
Undetermined.
  1. Lee S.D., 2008. Brevibacterium marinum sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58, 500-504.
Most characters are based on API Coryne and API ZYM results.

Positive results for casein hydrolysis, catalase, esterase (C4)  (weak reaction), esterase lipase (C8) (weak reaction), alpha-
glucosidase, beta-galactosidase, nitrate reduction, phosphoamidase & urease.

Negative results for acid phosphatase, alkaline phosphatase, arginine dihydrolase, cystine arylamidase, DNA hydrolysis, esculin
hydrolysis, alpha-fucosidase, gelatin hydrolysis, alpha-galactosidase, beta-glucosidase, beta-glucuronidase,
N-acetyl-beta-glucosaminidase, leucine arylamidase, lipase (C14), alpha-mannosidase, oxidase, pyrazinamidase, pyrrolidonyl
arylamidase, starch hydrolysis, trypsin, alpha-chymotrypsin, tyrosine decomposition, valine arylamidase, acid production from:
D-glucose, glycogen, lactose, maltose, mannitol, ribose, sucrose & xylose.
Brevibacterium marinum
(c) Costin Stoica
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