Phylum Actinobacteria, Class Actinobacteria, Order Micrococcales, Family Brevibacteriaceae, Genus Brevibacterium, Brevibacterium
linens (Wolff 1910) Breed 1953.
Historical synonyms: Bacterium linens Wolff 1910.
Gram-positive or Gram-negative relatively short rods with a marked rod-coccus cycle
when growing on complex media; young culture (up to 24 h) produce coryneform rods
with V arrangements, while older cells are coccoid or coccobacillary. Nonmotile.
Spores are not produced. Mycolic acids are not present.
Colonies are convex, smooth and shiny surface, small, 0.1-0.2 mm in diameter (1-2
days) to 2.5 mm (extended incubation) and pale yellow-orange on nutrient agar.
Pigment often produced only in the presence of light. Aerobic. CAMP test negative.
Nonfastidious. Halotolerant (10% NaCl). Can grow optimally at 20-25 ºC, weak and
variable growth at 5 and 37 ºC.
The habitats include surface-ripened cheeses (most common), raw milk, animal sources, water, soil and human sources.
- Funke G., von Graevenitz A., Clarridge III J.E., and Bernard K.A., 1997. Clinical Microbiology of Coryneform Bacteria. Clinical
Microbiology Rewiews Vol. 10, No. 1, p. 125-159.
- Funke G., 2006.Corynebacteria and rare coryneforms. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition,
Edited by Borriello S.P., Murray P.R. and Funke G.,Edward Arnold (Publishers) Ltd., Bacteriology, volume 2.
- Trujillo M.E. and Goodfellow M., 2012. Genus I. Brevibacterium Breed 1953, emend. Collins, Jones, Keddie and Sneath 1980.In:
Goodfellow M., Kampfer P., Busse H.J., Trujillo M.E., Suzuki K., Ludwig W. and Whitman W.B. (Editors), Bergey’s Manual of
Systematic Bacteriology, second edition, Vol. Five, The Actinobacteria, Part A, Springer, Athens, pp. 685-700.
- Collins M.D., Jones D., Keddie R.M. and Sneath P.H.A., 1980. Reclassification of Chromobacterium iodinum (Davis) in a
Redefined Genus Brevibacterium (Breed) as Brevibacterium iodinum nom.rev, ; comb.nov. J. Gen. Microbiol. 120, 1-10.
Positive results for casein hydrolysis, catalase, DNA hydrolysis, gelatin hydrolysis, hippurate hydrolysis, nitrate reduction, oxidase
(weak reaction) & pyrazinaminidase.
Negative results for esculin hydrolysis, alpha-glucosidase, lipase, starch hydrolysis, urease, acid production from: glucose, maltose,
mannitol, phenylacetate, sucrose & xylose.
Can utilize citrate, but not D-arabinose, gluconate or mannitol.
(c) Costin Stoica