|C. amycolatum, Gram stain
|C. amycolatum colonies on Sheep Blood Agar
Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order
Actynomycetales, Suborder Corynebacterinae, Family Corynebacteriaceae, Genus
Corynebacterium, Corynebacterium amycolatum Collins, Burton and Jones, 1988.
Historical synonyms: CDC coryneform groups F-2 and I-2.
Pleomorphic coryneform rods with single cells, V forms or "Chinese letters".
Very typically dry, waxy and grayish white with irregular edges colonies, 1.0-1.5 mm in
diameter after 24 hours of incubation at 37 ºC. CAMP test is negative.
Isolated from swabs of the skin of healthy people. Isolated from bovine milk (admin
note). Mostly O/129 resistant. May be multiresistant to beta-lactams, macrolides,
clindamycin, aminoglycosides, ciprofloxacin, and rifampin.
Susceptible to glycopeptides and, majority strains to tetracycline.
A postoperative sternal wound infection, a case of peritonitis associated with a
continuous ambulatory peritoneal dialysis and other opportunistic infections, foreign
body infections, bacteremia, urinary tract infections.
- Funke G., von Graevenitz A., Clarridge III J.E., and Bernard K.A., 1997. Clinical Microbiology of Coryneform Bacteria. Clinical
Microbiology Rewiews Vol. 10, No. 1, p. 125-159.
- Funke G ., 2006.Corynebacteria and rare coryneforms. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition,
Edited by Borriello S.P., Murray P.R. and Funke G.,Edward Arnold (Publishers) Ltd., Bacteriology, volume 2.
- Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T. and Williams S.T., 1994. Bergey's Manual of Determinative Bacteriology, Ninth
Edition, Williams & Wilkins, A Waverly Company, Baltimore, pp 571-596.
Fermentative metabolism. Non-partially-acid-fast.
Positive results for alkaline phosphatase, reduction of methyl red, pyrazinamidase,
acid production from: fructose, glucose & mannose.
Negative results for casein hydrolysis, esculin hydrolysis, mycolic acids, lipophilism,
tyrosine hydrolysis, acid production from: arabinose, galactose, lactose, mannitol,
raffinose, rhamnose, salicin, starch & xylose.
Variable results for hippurate hydrolysis, nitrate reduction, urease, acid production
from: maltose, sucrose & trehalose.
(c) Costin Stoica