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Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster I (Clostridium sensu
stricto),
Clostridium scatologenes  (Weinberg and Ginsbourg 1927) Prévot 1948.

Historical synonym:
 Clostridium scatol  Fellers and Clough 1925, Bacillus scatologenes  Weinberg and Ginsbourg 1927.
Gram-positive straight rods, 0.5-1.6 x 3.1-21.2 µm. Motile by peritrichous flagella.
Spores  are oval, terminal/subterminal, slightly swell the cell. The cell wall is
susceptible to dissolution by lysozyme.
Surface colonies on blood agar are alpha-hemolytic, 0.5-2.0 mm in diameter, circular,
convex, translucent, gray, with an entire or scalloped margin, a matt surface, and a
granular or mottled internal structure. Cultures in PYG broth are turbid with a smooth
sediment and have a pH of 5.2–5.4 after incubation for 5 days. Optimum temperature
for growth is 30-37 ºC; can grow at 25 but not at 45 ºC. Growth is inhibited by 6.5%
NaCl, pH 8.5 or 20% bile. Abundant gas is produced in PYG deep agar cultures. No
aero-tolerance. Products in PYG broth include large amounts of acetic and butyric
acids; abundant hydrogen is produced.
Isolated from soil, contaminated food, feces of infants undersized at birth.
Culture supernatants are not toxic for mice. Toxin is not produced. Nonpathogenic for laboratory animals.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. Smith L.D.S. and Hobbs G., 1974. Genus III. Clostridium Prazmowski 1880. In: (Eds.) Buchanan R.E. and Gibbons N.E., Bergey’s
    Manual of Determinative Bacteriology, Eighth Edition, The Williams & Wilkins Company, Baltimore, 551-572.
Milk reaction is negative. Meat is not digested.

Positive results for ammonia production, H
2S production, neutral red reduction, substrate utilization and/or acid production from:
fructose, glucose, glycerol & mannose.

Negative results for casein hydrolysis, gelatin hydrolysis, lecithinase, lipase, nitrate reduction, starch hydrolysis, urease,
Voges-Proskauer reaction, substrate utilization and/or acid production from: adonitol, amygdalin, cellulose, dulcitol, erythritol,
galactose, glycogen, inositol, inulin, lactose, maltose, mannitol, melezitose, melibiose, raffinose, sorbitol, sorbose, starch, sucrose &
trehalose.

Variable results for esculin hydrolysis, hippurate hydrolysis, indole production, resazurin reduction, substrate utilization and/or acid
production from: arabinose, cellobiose (weak), rhamnose, ribose, salicin (weak) & xylose.
Clostridium scatologenes
(c) Costin Stoica
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