Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster XIVa (non-Clostridium
sensu stricto), Clostridium saccharolyticum Murray, Khan and van den Berg 1982.
Gram-negative, spindle-shaped straight rods, 0.5-0.7 x 3.0 µm. Nonmotile; atrichous.
Spores are round, terminal/subterminal and distend the cell; sporulation occurs
readily in chopped-meat carbohydrate broth.
Surface colonies on cellobiose-yeast extract agar after 48 hours incubation are 0.5-
1.5 mm in diameter, circular with smooth margins, convex and white; on blood agar
plates, colonies are pinpoint - 1 mm in diameter, circular or slightly irregular, low
convex, with entire or slightly scalloped margins and a mottled or crystalline internal
structure. Cultures in PYG broth have a smooth sediment, with turbidity, and a pH of
5.1 after incubation for 4 days.
Optimum temperature for growth is 37 ºC. No growth at 14 or 45 ºC. Optimum pH 7.4,
Isolated from a methanogenic cellulose-enrichment culture from sewage sludge.
The type strain is susceptible to chloramphenicol, clindamycin, erythromycin, penicillin G, and tetracycline.
- N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
- Murray W.D., Khan A.W. and van den Berg L., 1982. Clostridium saccharolyticum sp. nov., a saccharolytic species from sewage
sludge. IJSB vol. 32, no.1, 132-135.
Milk reaction is positive (curd production). Meat is not digested.
Positive results for hydrogen production, ammonia production, esculin hydrolysis, H2S production, indole production, nitrate reduction,
resazurin reduction, starch hydrolysis, Voges-Proskauer reaction, substrate utilization and/or acid production from: adonitol,
arabinose, cellobiose, esculin, fructose, glucose, glycerol (weak), inositol, lactose, maltose, mannitol, mannose, melezitose,
melibiose, raffinose, rhamnose, ribose, salicin, sorbose, sucrose, trehalose & xylose.
Negative results for catalase, casein hydrolysis, gelatin hydrolysis, lecithinase, lipase, urease, substrate utilization and/or acid
production from: amygdalin, cellulose, dulcitol, erythritol, galactose, glycogen, inulin, sorbitol & starch.
(c) Costin Stoica
Abundant growth occurs in PY media supplemented with vitamin K and heme, with or without carbohydrate. There is no growth in a
defined carbohydrate-mineral salt-vitamin medium even when supplemented with synthetic mixtures of amino acids, purines, and
pyrimidines; good growth is obtained in this medium upon addition of yeast extract or in coculture with Acetivibrio cellulolyticus. In
minimal medium, 0.1% yeast extract, fermentable carbohydrate, iron, and a reduced form of sulfur are required for growth; growth in
this medium is enhanced by addition of B vitamins and phosphate.
Products in PYG broth are acetic, formic, and lactic acids. H2 and CO2 are produced.