Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster XI (Clostridium
non-sensu stricto), Clostridium mangenotii (Prévot and Zimmes-Chaverou 1947) McClung and McCoy 1957.
Historical synonyms: Inflabilis mangenoti Prévot and Zimmès-Chaverou 1947.
Gram-positive rods, 0.6-0.9 x 3.1-8.2 µm, occur singly/in pairs or in short chains
Nonmotile. Spores are oval, subterminal, swelling the cell; sporulation occurs most
readily on egg-yolk agar plates incubated for 72 hours.
Surface colonies on blood agar plates are pinpoint–1.0 mm, circular, low convex,
translucent, granular, gray-white, and dull, with an entire margin and a grainy surface.
Hemolytic. Cultures in PYG broth are turbid and have a stringy sediment and a pH of
6.2 after incubation for 7 days. Moderate to poor growth in nutrient or cooked meat
broth with stringy sediment; better growth in glucose broth.
Optimum temperature for growth is 30-37 ºC. Grow at 25 ºC, but not at 45 ºC. Growth
is inhibited by 6.5% NaCl and 20% bile. Moderate gas is detected in PYG deep agar
cultures. Products in PYG broth cultures include acetate, formate, isocaproate,
isovalerate, and isobutyrate; a large amount of H2 is formed.
Isolated from soil, marine sediments, human feces.
The type strain is susceptible to chloramphenicol, clindamycin, erythromycin, penicillin G, and tetracycline.
Supernatant cultures are nontoxic to mice. Nonpathogenic for laboratory animals. Toxin is not produced.
- N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
- Smith L.D.S. and Hobbs G., 1974. Genus III. Clostridium Prazmowski 1880. In: (Eds.) Buchanan R.E. and Gibbons N.E., Bergey’s
Manual of Determinative Bacteriology, Eighth Edition, The Williams & Wilkins Company, Baltimore, 551-572.
Milk reaction is variable. Meat digestion is positive. Hydrogen production is abundant.
Positive results for ammonia production, casein hydrolysis, gelatin hydrolysis, H2S production & indole production.
Negative results for esculin hydrolysis, lecithinase, lipase, nitrate reduction, urease, Voges-Proskauer reaction, acid production from:
amygdalin, arabinose, cellobiose, dulcitol, esculin, fructose, galactose, glucose, glycogen, glycerol, inositol, inulin, lactose, maltose,
mannitol, mannose, melezitose, melibiose, raffinose, rhamnose, ribose, salicin, sorbitol, sorbose, starch, sucrose, trehalose &
(c) Costin Stoica