Taxonomy
Morphology
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References
Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster I (Clostridium sensu
stricto),
Clostridium isatidis  Padden, Dillion, Edmonds, Collins, Alvarez and John 1999.

Description is based on a single isolate.
Gram-positive rods, 0.3-0.6 x 1.8-9.1 µm,  occuring singly or in pairs or chains. Motile.
Spores are oval, terminal, swelling the cell.
Colonies are mucoid, white, circular to irregular & convex or raised to a central point.
Slight beta-hemolysis occurred on horse blood agar but not on sheep blood agar.
Anaerobic, aerotolerant. Optimum temperature for growth is 49-52 ºC. Grow at 30-55
ºC. Optimum pH 7.2; range 5.6–9.9. No growth in 6.5% NaCl.
Growth on PYG produces acetic, lactic, and formic acids, and hydrogen.
Isolated from a woad vat.
Sensitive to tetracycline, gentamicin, penicillin, ampicillin, rifampicin. Resistant to chloramphenicol, kanamycin and erythromycin.
Undetermined.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. Padden A.N., Dillion V.M., Edmonds J., Collins M.D., Alvarez N. and John P., 1999. An indigo-reducing moderate thermophile from
    a woad vat, Clostridium isatidis sp. nov. IJSB 49, 1025-1031.
Milk reaction and meat digestion are negative.

Positive results for H
2 production, esculin hydrolysis, indigo reduction, substrate utilization and/or acid production from: amygdalin,
cellobiose, fructose, galactose, glucose, maltose, mannose, salicin, sorbitol, starch, sucrose & trehalose.

Negative results for catalase, gelatin hydrolysis, indole production, lecithinase, nitrate reduction, starch hydrolysis, urease, substrate
utilization and/or acid production from: arabinose, glycogen, inulin, lactose, mannitol, melezitose, raffinose, rhamnose, ribose &
xylose.
Clostridium isatidis
(c) Costin Stoica
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