Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster I (Clostridium sensu
stricto), Clostridium intestinale corrig. Lee, Fujisawa, Kawamura, Itoh and Mitsuoka 1989.
Gram-positive, straight to slightly curved rods, 0.3-0.4 x 1.4-5.4 µm, occuring singly or
in pairs/short chains. Motile by peritrichous flagella. Spores are large (0.1-1.1 x
1.5-2.0 µm), slightly oval, terminal, swelling the cell.
Colonies are circular, convex, rough, translucent, undulate, grayish-white, beta-
hemolytic, and 2–4 mm in diameter under anaerobic conditions. Under aerobic
conditions, colonies are smooth, yellowish white, and 1mm in diameter. Aerotolerant.
Optimum temperature for growth is 37 ºC. Fermentation products include large
amounts of acetic and lactic acids. Cultures in PYFG broth grow well and are turbid,
with a smooth, ropy, or flocculent sediment. Abundant gas is produced in PYFG deep-
Isolated from feces of cattle and pigs.
- N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
- Lee W.K., Fujisawa T., Kawamura S., Itoh K. and Mitsuoka T., 1989. Clostridium intestinalis sp. nov., an aerotolerant species
isolated from the feces of cattle and pigs. IJSB 39, No. 3, 334-336.
Positive results for H2 production, substrate utilization and/or acid production from:
amygdalin, cellobiose, fructose, galactose, glucose, lactose, mannitol, mannose, salicin, sorbitol, sucrose & trehalose.
Negative results for gelatin hydrolysis, H2S production, indole production, lecithinase, lipase, nitrate reduction, starch hydrolysis,
sulfate reduction, substrate utilization and/or acid production from: adonitol, arabinose, dulcitol, erythritol, esculin , glycogen, glycerol,
inositol, inulin, maltose, melezitose, melibiose, raffinose, rhamnose, ribose, sorbose, starch & xylose.
(c) Costin Stoica