Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster XIVa (Clostridium non
sensu stricto), Clostridium hylemonae Kitahara, Takamine, Imamura and Benno 2000.
Gram-positive, straight to slightly curved rods, 0.2-0.5 x 1.0-5.3 µm, occuring singly or
in pairs. Nonmotile. Can produce spores.
Obligate anaerobe. Colonies are 0.5-1.0 mm in diameter, disc-shaped, and gray.
Optimum temperature for growth is 37 ºC. Optimum pH 7.6. Acetic,butyric, propionic,
isobutyric and iso-valeric acid are produced as the end products in peptone-yeast-
extract medium supplemented with glucose.
Isolated from healthy human feces.
- N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
- Kitahara M., Takamine F., Imamura T. and Benno Y., 2000. Assignment of Eubacterium sp. VPI 12708 and related strains with
high bile acid 7α-dehydroxylating activity to Clostridium scindens and proposal of Clostridium hylemonae sp. nov., isolated from
human faeces. IJSEM 50, 971-978.
Positive for H2S production, substrate utilization and/or acid production from galactose,
glucose, raffinose & sucrose.
Negative results for esculin hydrolysis, gelatin hydrolysis, indole production, lipase, nitrate reduction, starch hydrolysis, substrate
utilization and/or acid production from: adonitol, amygdalin, cellobiose, dulcitol, erythritol, esculin, glycogen, glycerol, inositol, inulin,
lactose, mannitol, mannose, melezitose, melibiose, rhamnose, salicin, sorbitol, sorbose, starch & trehalose.
Variable results for substrate utilization and/or acid production from: arabinose, fructose, maltose, ribose & xylose.
(c) Costin Stoica