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Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster XI (Clostridium non
sensu stricto),
Clostridium hiranonis  Kitahara, Takamine, Imamura and Benno 2001.
Gram-positive, straight to slightly curved rods, 0.8 x 1.6-10.0 µm, occuring singly or in
pairs. Non-motile. Can produce spores.
Obligate anaerobe. Colonies on EG (Eggerth-Gagnon) blood agar are 1.0-2.0 mm in
diameter, disc-shaped, and gray to grayish-white. Optimum temperature for growth is
37 ºC. Can grow at 30 or 50 ºC. Optimum pH 7.5-8.0. Moderate amounts of acetic acid
and iso-valeric acid are produced as the end products in peptone-yeast-extract
medium supplemented with glucose.
Isolated from healthy human feces.
Undetermined.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. Kitahara M., Takamine F., Imamura T. and Benno Y., 2001. Clostridium hiranonis sp. nov., a human intestinal bacterium with bile
    acid 7a-dehydroxylating activity. IJSEM 51, 39-44.
Positive for substrate utilization and/or acid production from: fructose, glucose,
mannose & sucrose.

Negative results for alkaline phosphatase, catalase, esculin hydrolysis, gelatin hydrolysis, H
2S production, indole production, nitrate
reduction, starch hydrolysis, substrate utilization and/or acid production from: amygdalin, adonitol, arabinose, cellobiose, dulcitol,
erythritol, esculin, galactose, glycogen, glycerol, inositol, inulin, lactose, maltose, mannitol, melezitose, melibiose, raffinose,
rhamnose, ribose, salicin, sorbitol, sorbose, starch, trehalose & xylose.
Clostridium hiranonis
(c) Costin Stoica
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