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Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster IV (non-Clostridium
sensu stricto),
Clostridium cellulosi  He, Ding and Long 1991.
Gram-negative, straight or slightly curved rods, 0.3-0.6 x 2.0-15.0 µm, occur singly or
in pairs/chains. Motile by lophotrichous flagella. Spores are sphaerical, terminal,
swelling the cells.
Strict anaerobe. Colonies show clear zones on cellulose agar after 48 hours, and
continue expansion afterwards. Tiny, white colonies appear within centers of the clear
zone. Surface colonies are watery, irregular, and spread. Deep colonies are round
with entire margins. Grows on 2g of yeast / liter. Slight growth occurs in PY medium
(peptone, yeast extract) with no fermentable carbohydrate. Grows at 40-65 ºC and pH
6.2-8.5. Optimum temperature 55-60 ºC; optimum pH 7.3-7.5.
Isolated from cow manure compost. Cultures can survive 100 ºC for 20 minutes.
Undetermined.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. He Y., Ding Y., and Long Y., 1991. Two Cellulolytic Clostridium Species: Clostridium cellulosi sp. nov. and Clostridium
    cellulofermentans sp. nov. IJSB 41, No. 2, p. 306-309.
Cellulose is fermented to carbon dioxide, hydrogen, ethanol and acetic acid.
Milk reaction is positive (curd).

Positive results for hydrogen production, esculin hydrolysis, Voges-Proskauer test, substrate utilized and/or acid produced from:
cellobiose, cellulose, fructose, galactose, glucose, glycogen, inositol, inulin, lactose, maltose, mannitol, mannose, melibiose,
raffinose, salicin, starch, sucrose, trehalose & xylose.

Negative results for catalase, indole production, gelatin hydrolysis, nitrate reduction, substrate utilized and/or acid produced from:
arabinose, erythritol, glycerol, melezitose, rhamnose, sorbitol & sorbose.
Clostridium cellulosi
(c) Costin Stoica
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