Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster I (Clostridium sensu
stricto),
Clostridium celatum  Hauschild and Holdeman 1974.
Gram-positive straight or curved rods, 0.8-3.0 x 6.3 µm, occuring singly, in pairs or in chains, filaments. Nonmotile. Spores are oval,
terminal/subterminal/central, usually not swelling the cell; sporulation occurs most
readily on solid media (blood agar or egg-yolk agar plates or chopped-meat slants)
Surface colonies on blood agar plates are 2-7 mm in diameter, lobate to erose, flat to
low convex, translucent to opaque, white, with a granular or mottled internal structure.
Some strains were alpha-hemolytic, some were non
-haemolytic on rabbit blood.
Cultures in PYG broth are turbid with a smooth or fluffy sediment and have a pH of 5.2-
5.5  after incubation for 1 week. Growth is stimulated by fermentable carbohydrates.
Growth is inhibited by 6.5% NaCl and 20% bile.

Grows optimally at 37 ºC. Variable, weak growth at 25 or 45 ºC. Products in PYG
broth:  acetic and formic acids, ethanol  and hydrogen. Slight to moderate gas is
produced in PYG deep agar cultures.
Isolated from human feces. Susceptible to chloramphenicol, clindamycin, penicillin G  and tetracycline.
Nonpathogenic for laboratory animals.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
H2 is produced very abundantly. Milk reaction is positive (acidified and slightly curdled).

Positive results for esculin hydrolysis, H
2S production from bisulfite not from sulphate, neutral red reduction, urease, substrate utilized
and/or acid produced from: amygdalin, cellobiose, fructose, galactose, lactose, maltose, mannose, salicin, sucrose & trehalose.

Negative results for indole production, lecithinase, lipase, starch hydrolysis, resazurin reduction, substrate utilized and/or acid
produced from: arabinose, glycogen, inulin, mannitol, melezitose, melibiose, raffinose, rhamnose, sorbitol, starch & xylose.

Variable results for nitrate reduction, substrate utilized and/or acid produced from ribose.
Clostridium celatum
(c) Costin Stoica
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