Taxonomy
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Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster XIVa (non-Clostridium
sensu stricto),
Clostridium bolteae  Song, Liu, Molitoris, Tomzynski, Lawson, Collins and Finegold 2003.
Gram-positive rods, 1.0-1.2 x 2.0-5.0 µm, that form occasional subterminal spores.
Obligately anaerobic. Colonies on Brucella blood agar plates are 2–3 mm in diameter,
slightly raised, circular with slighly scalloped edges, dull, opaque and smooth. Grow at
37 ºC.
Isolated from human gut, human faecal material, blood and intra-abdominal abscess.
Susceptible to kanamycin and colistin sulphate. Resistant to ampicillin.
Undetermined. It is believed that may be responsible for gastrointestinal issues prevalent in 90 percent of autistic children.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. Helena MRT Parracho, Max O Bingham, Glenn R Gibson and Anne L McCartney. Differences between the gut microflora of
    children with autistic spectrum disorders and that of healthy children. J Med Microbiol October 2005 vol. 54 no. 10 987-991.
Saccharolytic. Positive results for substrate utilized and/or acid produced from:
arabinose, fructose, glucose, glycerol, maltose, mannose, melezitose, sorbitol,
sucrose, trehalose & xylose.

Negative results for esculin hydrolysis, gelatin hydrolysis, indole production,
lecithinase, lipase, nitrate reduction, urease, substrate utilized and/or acid produced
from: erythritol, inulin, lactose, mannitol, melibiose, ribose & salicin.
Clostridium bolteae
(c) Costin Stoica
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