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Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster I (Clostridium sensu
stricto),
Clostridium aurantibutyricum  Hellinger 1944.
Gram-positive (Gram-negative in older cultures), straight rods, 0.5-0.8 x 2.8-6.3 µm,
occurring singly and in pairs. Motile by peritrichous flagella. Spores are oval,
subterminal, swelling the cell; sporulation occurs most readily on chopped-meat agar
slants incubated at 30 ºC for 1 week.
Surface colonies on blood agar plates are 1–2 mm in diameter, circular to slightly
irregular, raised to  low convex, translucent, gray to pink-orange, dull, smooth, with a
mosaic internal structure. Blood is hemolysed.
Slight growth in broth with or without fermentable carbohydrate. Growth is inhibited by
6.5% NaCl and by 20% bile. Optimum growth at:  37 ºC and pH 7.0. No growth at 25 or
45 ºC.
PYG broth cultures are turbid with a heavy, ropy, or viscous sediment and have a pH of
5.4 after incubation for 6 days. Abundant gas is detected in PYG deep agar cultures.
Major products of metabolism in PYG broth: acetate and lactate.
Isolated from rotting hibiscus stumps, flax, rotting potatoes, soil, sewage sludge; bovine, human infant, and adult feces.
Susceptible to chloramphenicol, clindamycin, erythromycin, penicillin G, and tetracycline.
Nonpathogenic for laboratory animals (mouse). Toxin is not produced.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. Smith L.D.S. and Hobbs G., 1975. Genus III. Clostridium Prazmowski 1880. In: (Eds.) Buchanan R.E. and Gibbons N.E., Bergey’s
    Manual of Determinative Bacteriology, Eighth Edition , The Williams & Wilkins Company, Baltimore, 551-572.
H2 is produced in large amounts. Milk reaction is positive (stormy fermentation; a solid curd is formed with 50% digestion in 3 weeks).
Meat digestion is negative.

Positive results for H
2 production, esculin hydrolysis, gelatin hydrolysis, lipase, neutral red reduction, nitrate reduction, resazurin
reduction, starch hydrolysis, substrate utilized and/or acid produced from: arabinose (weak), cellobiose, dulcitol, fructose, galactose
(weak), glucose, glycogen, lactose, maltose, mannose (weak), melibiose (weak), raffinose, salicin (weak), sorbose, starch (weak) &
sucrose.

Negative results for casein hydrolysis, H
2S production, indole production, lecithinase, urease, Voges-Proskauer reaction, substrate
utilized and/or acid produced from: amygdalin, glycerol, inositol, inulin, mannitol, melezitose, rhamnose, ribose, sorbitol, trehalose &
xylose.
Clostridium aurantibutyricum
(c) Costin Stoica
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