Colonies on R2A agar after 48h incubation at 25 ºC, are round, entire, convex and
pale-yellow-pigmented, 0.8–1.0 mm in diameter. Growth occurs at 15-42 ºC, optimally
at 25-35 ºC. Can grow in pH 6.0-8.0, optimally in pH 7.0-8.0. Can grow in 0-1% (w/v)
NaCl, optimally in 0% NaCl. Aerobic.
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Pseudogulbenkiania,
Pseudogulbenkiania subflava Lin et al. 2008, type species of the genus.
Gram-negative rods, 0.2-0.5 x 1.0-1.6 μm. Motile by means of a polar flagellum.
Spores are not formed. Accumulate poly-beta-hydroxybutyrate.
Isolated from a water sample of a cold spring located in the Hsinchu area of northern Taiwan.
Sensitive to tetracycline, kanamycin, rifampicin, gentamicin, penicillin G, chloramphenicol, nalidixic acid, novobiocin, streptomycin,
ampicillin and sulfamethoxazole + trimethoprim.
Undetermined.
  1. Lin M.C., Chou J.H., Arun A.B., Young C.C. and Chen W.M.: Pseudogulbenkiania subflava gen. nov., sp. nov., isolated from a cold
    spring. Int. J. Syst. Evol. Microbiol., 2008, 58, 2384-2388.
  2. Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
    1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
Positive results for acid and alkaline phosphatase, C4 esterase, C8 esterase lipase,
alpha-glucosidase, leucine arylamidase, naphthol-AS-BIphosphohydrolase, nitrate reduction & oxidase.
Can utilize alpha-D-glucose, maltose, D-gluconate, caprate, malate and citrate.
Can utilize in Biolog GN: Tween 80, alpha-D-glucose, maltose, sucrose, trehalose, turanose, pyruvic acid methyl ester, succinic acid
monomethyl ester, formic acid, D-gluconic acid, DL-lactic acid, quinic acid, succinic acid, bromosuccinic acid, L-alanine,
L-asparagine, L-aspartic acid, L-glutamic acid, L-histidine, hydroxy-L-proline, L-leucine, L-ornithine, L-proline, DL-carnitine,
gamma-aminobutyric acid & urocanic acid.

Negative results for arginine dihydrolase, cystine arylamidase, alpha-fucosidase, beta-glucosidase, alpha- and beta-galactosidase,
beta-glucuronidae, indole production, C14 lipase, alpha-mannosidase, N-acetyl-beta-glucosaminidase, nitrite reduction to N2,
protease,  trypsin, alpha-chymotrypsin, urease, valine arylamidase, acid production from amygdalin, L-arabinose, D-glucose,
glycogen, myo-inositol, alpha-D-lactose, maltose, D-mannitol, melibiose, L-rhamnose, ribose, D-sorbitol, sucrose & xylose.
No utilization of arabinose, mannose, mannitol, N-acetylglucosamine, adipate & phenylacetate.
Cannot utilize in Biolog GN: alpha-cyclodextrin, dextrin, glycogen, Tween 40, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine,
adonitol, L-arabinose, D-arabitol, cellobiose, i-erythritol, D-fructose, L-fucose, D-galactose, gentiobiose, myo-inositol,
alpha-D-lactose, lactulose, D-mannitol, D-mannose, melibiose, methyl beta-D-glucoside, D-psicose, raffinose, L-rhamnose,
D-sorbitol, xylitol, acetic acid, cis-aconitic acid, citric acid, D-galactonic acid lactone, D-galacturonic acid, D-glucosaminic acid,
D-glucuronic acid, alpha-hydroxybutyric acid, beta-hydroxybutyric acid, gamma-hydroxybutyric acid, p-hydroxyphenylacetic acid, itaconic
acid, alpha-ketobutyric acid, alpha-ketoglutaric acid, alpha-ketovaleric acid, malonic acid, propionic acid, D-saccharic acid, sebacic
acid, succinamic acid, glucuronamide, L-alaninamide, D-alanine, L-alanyl glycine, glycyl L-aspartic acid, glycyl L-glutamic acid,
L-phenylalanine, L-pyroglutamic acid, D-serine, L-serine, L-threonine, inosine, uridine, thymidine, phenylethylamine, putrescine,
2-aminoethanol, 2,3-butanediol, glycerol, DL-alpha-glycerol phosphate, alpha-D-glucose 1-phosphate, or D-glucose 6-phosphate.
Pseudogulbenkiania subflava
(c) Costin Stoica
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