Colonies on nutrient agar after 48h incubation at 32 ºC are small, round, entire,
convex, white and 1.0-1.3 mm in diameter.
Growth occurs at 25-40 ºC, optimally at 32
ºC. Can grow in pH 6
.0-8.0, optimally in pH 6.0. Can grow in 0.3-2% (w/v) NaCl,
optimally in 0.7% NaCl
. Aerobic.
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Pseudogulbenkiania,
Pseudogulbenkiania gefcensis Lee et al. 2013.
Gram-negative, short rods, 0.3-0.5 x 0.6-0.8 μm. Motile by means of a polar flagellum.
Spores are not formed.
Isolated from soil samples in the Republic of Korea.
Susceptible to ampicillin (25 μg), tetracycline (30 μg), kanamycin (30 μg), rifampicin (5 μg), gentamicin (30 μg), chloramphenicol (30
μg), novobiocin (30 μg), sulfamethoxazole + trimethoprim (25 μg) and streptomycin (25 μg).
Undetermined.
  1. Lee D.G., Im D.M., Kang H., Yun P., Park S.K., Hyun S.S. and Hwang D.Y.: Pseudogulbenkiania gefcensis sp. nov., isolated from
    soil. Int. J. Syst. Evol. Microbiol., 2013, 63, 187-191.
  2. Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
    1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
Positive results for nitrate reduction & oxidase.
Can utilize (Biolog GN2) dextrin, glycogen, Tween-80, D-fructose, succinic acid monomethyl ester, formic acid, beta-hydroxybutyric
acid, p-hydroxyphenylacetic acid, DL-lactic acid, quinic acid, D-saccharic acid, succinic acid, bromosuccinic acid, L-alanine, L-
asparagine, L-aspartic acid, L-glutamic acid, L-histidine, hydroxy-L-proline, L-ornithine, L-phenylalanine, L-proline, gamma-
aminobutyric acid & urocanic acid.

Negative results for arginine dihydrolase,
catalase, citrate utilization, indole production, beta-glucosidase, protease, beta-
galactosidase, urease,
acid production from D-glucose.
No utilization of alpha-cyclodextrin, Tween-40, Nacetyl-D-galactosamine, N-acetyl-D-glucosamine, adonitol, L-arabinose, D-arabitol,
D-cellobiose, i-erythritol, L-fucose, D-galactose, gentiobiose, myo-inositol, alpha-D-lactose, lactulose, D-mannitol, D-mannose, D-
melibiose, methyl beta-Dglucoside, D-psicose, D-raffinose, L-rhamnose, D-sorbitol, sucrose, D-trehalose, turanose, xylitol, acetic
acid, cisaconitic acid, citric acid, D-galactonic acid lactone, D-galacturonic acid, D-glucosaminic acid, D-glucuronic acid, alpha-
hydroxybutyric acid, gamma-hydroxybutyric acid, itaconic acid, alpha-ketobutyric acid, alpha-ketoglutaric acid, alpha-ketovaleric acid,
malonic acid, propionic acid, D-saccharic acid, sebacic acid, succinamic acid, glucuronamide, L-alaninamide, Dalanine, L-alanyl
glycine, glycyl L-aspartic acid, glycyl Lglutamic acid, L-leucine, L-pyroglutamic acid, D-serine, L-serine, L-threonine, DL-carnitine,
inosine, uridine, thymidine, phenylethylamine, putrescine, 2-aminoethanol, 2,3-butanediol, glycerol, DL-a-glycerol phosphate, alpha-
D-glucose, 1-phosphate or D-glucose 6-phosphate.
Pseudogulbenkiania gefcensis
(c) Costin Stoica
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