Colonies on R2A medium are brown to purple, circular, convex and with entire
margins. Growth occurs at 20-37 ºC (optimum, 30 ºC), at pH 6-8 (optimum, pH 7.0)
and in R2A broth supplemented with 0-1.5% NaCl (w/v, optimum, 0% NaCl).
Facultatively anaerobic. Good growth occurs on R2A, NA and TSA, but not on MA,
blood, or MacConkey agars.
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Paludibacterium,
Paludibacterium purpuratum Kang et al. 2016.
Gram-negative, curved rods, 0.5-0.7 x 1.8-2.5 µm. Motile by means of a single polar
Isolated from a wetland in Jeju island, Republic of Korea.
Susceptible to chloramphenicol, kanamycin, rifampicin, streptomycin and tetracycline. Resistant to ampicillin, erythromycin,
gentamicin, penicillin G and vancomycin.
- Kang, H., Kim, H., Joung, Y., Kim, K. J. and Joh, K. 2016. Paludibacterium purpuratum sp. nov., isolated from wetland soil. Int. J.
Syst. Evol. Microbiol., 66, 2711-2716.
- Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
Description is based on API 20NE, API ZYM and Biolog GN tests.
Positive results for acid and alkaline phosphatase, arginine dihydrolase, casein (skimmed milk) hydrolysis, esculin hydrolysis,
esterase (C4), esterase lipase (C8), gelatin hydrolysis, alpha-glucosidase leucine arylamidase, naphthol-AS-BI-phosphohydrolase &
acid production from glucose.
Can utilize: D-glucose, L-arabinose, N-acetyl-D-glucosamine, potassium gluconate, malic acid, Tween 40, Tween 80, D-fructose,
D-trehalose, pyruvic acid methyl ester, D,L-lactic acid, bromosuccinic acid, L-alaninamide, L-alanine, L-alanyl-glycine, L-asparagine,
L-aspartic acid, L-glutamic acid, glycyl-Laspartic acid, glycyl-L-glutamic acid, L-proline, urocanic acid, alpha-D-glucose-1-phosphate &
Negative results for CM-cellulose hydrolysis cellulose (filter paper) hydrolysis, cystine arylamidase, dextrin hydrolysis, DNase,
alpha-fucosidase, alpha- and beta-galactosidase, beta-glucuronidase, beta-glucosidase, N-acetyl-beta-glucosaminidase, indole
production, lipase (C14), alpha-mannosidase, nitrate reduction, nitrite reduction, starch hydrolysis, trypsin, alpha-chymotrypsin,
urease & valine arylamidase.
No utilization of: D-mannose, D-mannitol, D-maltose, sucrose, capric acid, adipic acid, trisodium citrate, phenylacetic acid,
alpha-cyclodextrin, N-acetyl-D-galactosamine, adonitol, L-arabinose, D-arabitol, D-cellobiose, i-erythritol, L-fucose, D-galactose,
gentiobiose, m-inositol, alpha-D-lactose, lactulose, D-melibiose, beta-methyl-D-glucoside, D-psicose, D-raffinose, L-rhamnose,
D-sorbitol, turanose, xylitol, succinic acid mono-methyl-ester, cis-aconitic acid, citric acid, formic acid, D-galactonic acid lactone,
D-galacturonic acid, D-gluconic acid, D-glucosaminic acid, D-glucuronic acid, ghydroxybutyric acid, p-hydroxy phenylacetic acid,
itaconic acid, alpha-keto glutaric acid, alpha-keto valeric acid, malonic acid, quinic acid, D-saccharic acid, sebacic acid, succinic acid,
succinamic acid, glucuronamide, L-histidine, hydroxyl-L-proline, L-leucine, L-ornithine, L-phenylalanine, L-pyroglutamic acid,
D-serine, L-threonine, D,L-carnithine, inosine, uridine, phenylethyl-amine, putrescine, 2-aminoethanol, 2,3-butanediol, glycerol,
D,L-alpha-glycerol phosphate, dextrin, glycogen, acetic acid, alpha-hydroxybutyric acid, beta-hydroxybutyric acid, propionic acid,
D-alanine, L-serine, gamma-amino butyric acid, thymidine & alpha-keto butyric acid.
(c) Costin Stoica