Colonies are smooth, shiny and convex with spreading edges, 1.0-2.0 mm in
diameter, and are non-fluorescent. A violet pigment is produced. The optimal
temperature for growth is 32 ºC. The optimal pH for growth is 6.0; growth occurs
at pH 5 and 9.5, but not at pH 4.0 or 10. Growth occurs in the presence of 0-3.5% (w/v)
NaCl; optimum in 0-0.1% (w/v) NaCl. Good growth occurs after 48 h incubation on
tryptone soy agar (TSA) and nutrient agar (NA) at 32 ºC. Aerobic. No anaerobic growth
in the presence of nitrate.
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Chromobacterium,
Chromobacterium piscinae Kampfer et al. 2009.
Gram-negative rods, 0.3-0.5 / 1.5–2 μm. Motile.
Isolated from pond water.
- Kampfer P, Busse H, Scholz H. 2009. Chromobacterium piscinae sp. nov. and Chromobacterium pseudoviolaceum sp. nov., from
environmental samples Int J Syst Evol Microbiol 59(10):2486-2490 doi:10.1099/ijs.0.008888-0.
- Bajaj A, Kumar A, Yadav S, Kaur G, Bala M, Singh N, Mathan Kumar R, Manickam N, Mayilraj S. 2016. Isolation and
characterization of a novel Gram-negative bacterium Chromobacterium alkanivorans sp. nov., strain IITR-71T degrading
halogenated alkanes. Int J Syst Evol Microbiol 66(12):5228-5235 doi:10.1099/ijsem.0.001500.
- Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
Can be differentiated from C. pseudoviolaceum by positive utilization of cis-aconitate, propionate & DL-3-hydroxybutyrate.
Positive results for nitrate reduction to nitrite, gamma-glutamyl transferase, H2S production, tyrosine arylamidase, urease, oxidase
(negative in Bajaj's study), acid production from: fructose, glucose, mannose & trehalose.
Can utilize: L-aspartate, L-proline, N-acetyl-D-glucosamine, D-fructose, D-glucose, gluconate, D-ribose, trehalose, fumarate, acetate,
DL-lactate, L-malate, pyruvate, L-alanine, beta-alanine, L-histidine & L-serine.
Negative results for catalase (positive in Bajaj's study), esculin hydrolysis, gelatin liquefaction, alpha- and beta-galactosidase, alpha-
and beta-glucosidase, beta-glucuronidase, indole production, methyl red reduction, Voges-Proskauer test, acid production from:
adonitol, L-arabinose,D-arabitol, cellobiose, dulcitol, erythritol, inositol, lactose, D-mannitol, melibiose, D-mannose, maltose, methyl
D-glucoside, raffinose, L-rhamnose, salicin, sucrose, sorbitol & xylose.
No utilization of: citrate (positive in Bajaj's study), myo-inositol, D-mannitol, D-sorbitol, cellobiose, D-galactose, D-mannose,
melibiose, L-rhamnose, sucrose, salicin, D-xylose, adonitol, maltitol, L-leucine, putrescine, N-acetyl-D-galactosamine,
trans-aconitate, adipate, 4-aminobutyrate, azelate, glutarate, itaconate, mesaconate, suberate, L-ornithine, L-phenylalanine,
L-tryptophan, phenylacetate, oxoglutarate, 3- and 4-hydroxybenzoate.
(c) Costin Stoica