Colonies on CB medium display a large clear zone around them, derived from
degradation of chitin. No diffusible pigments are produced. Grow well by using chitin
as the sole carbon, nitrogen and energy source. Aerobic and facultatively anaerobic.  
Growth occurs at 15-40 ºC. Grows at pH 7.0-11.0 and with 0-0.75% NaCl.
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Chitinilyticum,
Chitinilyticum litopenaei Chang et al. 2009.
Gram-negative rods, 0.3-0.5 x 3.0-4.5 μm. Motile by a single polar flagellum. Poly-beta-
hydroxybutyrate granules are accumulated. Nonsporeforming.
Isolated from surface water of an aquaculture pond containing Pacific white shrimp (Litopenaeus vannamei) at Pingtung, Taiwan.
Susceptible to amikacin, ampicillin, chloramphenicol, kanamycin, nalidixic acid, novobiocin, rifampicin and tetracycline.
Resistant to erythromycin, gentamicin, penicillin G and streptomycin.
  1. Chang S.C., Wu M.C., Chen W.M., Tsai Y.H. and Lee T.M.: Chitinilyticum litopenaei sp. nov., isolated from a freshwater shrimp
    pond, and emended description of the genus Chitinilyticum. Int. J. Syst. Evol. Microbiol., 2009, 59, 2651-2655.
  2. Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
    1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature.
Most results are based on  API 20 NE, API ZYM and Biolog GN2.

Positive results for acid and alkaline phosphatase, catalase, chitin hydrolysis, nitrate reduction, C4 esterase, leucine arylamidase
Can assimilate: mannose, N-acetyl-D-glucosamine & gluconate.
Oxidation in Biolog GN2: dextrin, glucose, D-fructose, glycogen,
maltose, sucrose, cellobiose, mannose, trehalose, turanose,
N-acetyl-D-glucosamine, methyl pyruvate and gentiobiose.

Negative results for arginine dihydrolase,
C8 lipase, C14 lipase, esculin hydrolysis, alpha-fucosidase, alpha- and beta-
galactosidase, alpha- and beta-glucosidase, beta-glucuronidase, gelatin hydrolysis, indole production
, alpha-mannosidase, oxidase,
trypsin and alpha-chymotrypsin, urease, valine arylamidase, cystine arylamidase, acid production from: glucose.
No assimilation of: arabinose, mannitol, maltose, caprate, adipate, malate, citrate and phenylacetate.
No utilization in Biolog GN2:
gluconate, D-glucuronic acid, acetic acid, alpha-ketobutyric acid, alpha-ketovaleric acid, DL-lactate, alpha-
hydroxybutyric acid, glucose 6-phosphate, alphs-cyclodextrin, Tweens 40 and 80, arabinose, lactulose, psicose, melibiose, itaconic
acid, L-fucose, inosine, alpha-lactose, D-galactose, raffinose, L-rhamnose, mannitol, xylitol, glycerol, arabitol, i-erythritol, D-
galacturonic acid, D-sorbitol, myo-inositol, adonitol, 2,3-butanediol, N-acetyl-D-galactosamine, DL-alpha-glycerol phosphate, glucose
1-phosphate, methyl beta-D-glucoside, 2-aminoethanol, monomethyl succinate, citrate, formic acid, D-galactonic acid lactone,
malonic acid, aketoglutaric acid, propionic acid, urocanic acid, D-saccharic acid, succinic acid, bromosuccinic acid, succinamic acid,
phydroxyphenylacetic acid, cis-aconitic acid, quinic acid, sebacic acid, gamma-aminobutyric acid, beta- and gamma-hydroxybutyric
acids, D-glucosaminic acid, phenylethylamine, L-glutamic acid, L-histidine, L-aspartic acid, L-leucine, L-threonine, Lphenylalanine, L-
asparagine, L- and D-alanine, L- and D-serine, L-proline, hydroxy-L-proline, glycyl L-glutamic acid, L-ornithine, DL-carnitine, L-
pyroglutamic acid, glycyl Laspartic acid, L-alanyl glycine, putrescine, alaninamide, glucuronamide, thymidine & uridine.
Chitinilyticum litopenaei
(c) Costin Stoica
Culture media
Biochemical tests
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