Colonies on CB medium display a large clear zone around them, which is derived
from degradation of chitin. No diffusible pigments are produced. Grow well by using
chitin as the sole carbon, nitrogen and energy source. Aerobic and facultatively
anaerobic. Growth occurs at 15-40 ºC. Grows at pH 7.0-11.0 and with 0-0.75% NaCl.
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Chitinilyticum,
Chitinilyticum aquatile Chang et al. 2007 - type species of the genus.
Gram-negative rods, 0.3-0.4 x 2.5-4.0 μm. Motile by a single polar flagellum. Poly-beta-
hydroxybutyrate granules are accumulated. Nonsporeforming.
Isolated from surface water of an aquaculture pond containing Pacific white shrimp (Litopenaeus vannamei) at Pingtung, Taiwan.
Susceptible to amikacin, ampicillin, chloramphenicol, kanamycin, nalidixic acid, novobiocin, rifampicin and tetracycline.
Resistant to erythromycin, gentamicin, penicillin G and streptomycin.
- Chang S.C., Chen W.M., Wang J.T. and Wu M.C.: Chitinilyticum aquatile gen. nov., sp. nov., a chitinolytic bacterium isolated from a
freshwater pond used for Pacific white shrimp culture. Int. J. Syst. Evol. Microbiol., 2007, 57, 2854-2860.
- Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
Most results are based on API 20 NE, API ZYM and Biolog GN2.
Positive results for acid and alkaline phosphatase, catalase, chitin hydrolysis, gelatin hydrolysis, nitrate reduction, C4 esterase, C8
lipase, leucine arylamidase, oxidase & acid production from glucose.
Can assimilate: glucose, mannose, N-acetyl-D-glucosamine & gluconate.
Oxidation in Biolog GN2: dextrin, glucose, D-fructose, sucrose, cellobiose, mannose, trehalose, turanose, gluconate, D-glucuronic
acid, acetic acid, alpha-ketobutyric acid, alpha-ketovaleric acid, DL-lactate, N-acetyl-D-glucosamine, alpha-hydroxybutyric acid,
glucose 6-phosphate & L-alanine.
Negative results for arginine dihydrolase, C14 lipase, esculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase, alpha-
and beta-glucosidase, beta-glucuronidase, H2S production (TSI & SIM), indole production, lysine decarboxylase, alpha-mannosidase,
ornithine decarboxylase, trypsin and alpha-chymotrypsin, urease, Voges-Proskauer test, valine arylamidase, cystine arylamidase,
acid production from: mannitol, sucrose, arabinose & rhamnose.
No assimilation of: arabinose, mannitol, maltose, caprate, adipate, malate, citrate and phenylacetate.
No utilization in Biolog GN2: alpha-cyclodextrin, glycogen, Tween 40, Tween 80, arabinose, maltose, lactulose, psicose, D-melibiose,
itaconic acid, L-fucose, inosine, alpha-D-lactose, D-galactose, D-raffinose, gentiobiose, L-rhamnose, mannitol, xylitol, glycerol,
arabitol, i-erythritol, D-galacturonic acid, D-sorbitol, myo-inositol, adonitol, 2,3-butanediol, N-acetyl-D-galactosamine,
DL-alpha-glycerol phosphate, glucose 1-phosphate, methyl beta-D-glucoside, 2-aminoethanol, monomethyl succinate, citrate, formic
acid, D-galactonic acid lactone, methyl pyruvate, malonic acid, alpha-ketoglutaric acid, propionic acid, urocanic acid, D-saccharic acid,
succinic acid, bromosuccinic acid, succinamic acid, phydroxyphenylacetic acid, cis-aconitic acid, quinic acid, sebacic acid,
gamma-aminobutyric acid, beta-hydroxybutyric acid, gamma-hydroxybutyric acid, D-glucosaminic acid, phenyl ethylamine, L-glutamic
acid, L-histidine, L-aspartic acid, L-leucine, L-threonine, L-phenylalanine, L-asparagine, L-serine, D-alanine, D-serine, L-proline,
hydroxy-L-proline, glycyl L-glutamic acid, L-ornithine, DL-carnitine, L-pyroglutamic acid, glycyl L-aspartic acid, L-alanyl glycine,
putrescine, alaninamide, glucuronamide, thymidine & uridine.
(c) Costin Stoica