Colonies on R2A agar after 48 h incubation at 25 ºC are milky white, circular and
slightly convex with entire edges, 0.7-1.2 mm in diameter. Strictly aerobic. Growth
occurs at 10-37 ºC (optimum 25-30 ºC), at pH 6.0-8.0 (optimum pH 6.0-7.0) and with
0-2% NaCl (optimum 0-1% NaCl).
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Chitinibacter,
Chitinibacter fontanus Sheu et al. 2016.
Gram-negative rods, 0.4-0.6 x 0.9-1.4 μm. Motile by a single polar flagellum. Poly-
beta-hydroxybutyrate accumulation is observed.
Isolated from a spring in the vicinity of Miaoli County, Taiwan.
Sensitive to chloramphenicol, kanamycin, nalidixic acid, novobiocin, rifampicin, sulphamethoxazole + trimetoprim, tetracycline,
streptomycin, gentamicin and resistant to ampicillin and penicillin G.
- Sheu, S. Y., LI, Y. S., Young, C. C. and Chen, W. M. 2016. Chitinibacter fontanus sp. nov., isolated from a spring. Int. J. Syst. Evol.
Microbiol., 66, 4262-4268.
- Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
Most results are based on API 20 NE, API ZYM and Biolog GN2.
Positive results for acid and alkaline phosphatase, catalase, chitin hydrolysis, casein hydrolysis, cystine arylamidase, esterase (C4),
esterase lipase (C8), alpha-glucosidase, N-acetyl-beta-glucosaminidase, leucine arylamidase, nitrate reduction, oxidase, Tweens
40, 60 and 80 hydrolysis, acid production from: D-ribose, D-glucose, D-fructose, D-mannose, aesculin, maltose, D-lyxose,
N-acetylglucosamine, D-tagatose, gluconate and 5-ketogluconate.
Can assimilate glucose & mannose.
Oxidize (Biolog GN2): N-acetyl-D-glucosamine, D-fructose, alpha-D-glucose, D-mannose, D-gluconic acid, dextrin, maltose, pyruvic
acid methyl ester, alpha-ketobutyric acid, L-alaninamide, D-alanine, L-alanine, L-alanyl-glycine & L-serine.
Negative results for arginine dihydrolase, DNase, esculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase,
beta-glucuronidase, beta-glucosidase, gelatin hydrolysis, indole production, lipase, lipase (C14), alpha-mannosidase, starch
hydrolysis, Tween 20 hydrolysis, trypsin, alpha-chymotrypsin, urease & valine arylamidase.
No assimiliation of: arabinose, mannitol, maltose, caprate, adipate, malate, citrate & phenylacetate.
No oxidation of: glycogen, Tween 40 and 80, citric acid, malonic acid, succinic acid, L-asparagine, L-aspartic acid, L-glutamic acid,
glycyl-L-aspartic acid, glycyl-L-glutamic acid, L-histidine, L-phenylalanine, D-serine & glycerol.
(c) Costin Stoica