Colonies on R2A agar after 72 h incubation at 20 ºC are milky white, circular and
slightly convex with entire edges, 1.0–2.0 mm in diameter. Strictly aerobic. Growth
occurs at 10-35 ºC (optimum 20 ºC), at pH 6.0-8.0 (optimum pH 7) and with 0-1%
NaCl (optimum 0% NaCl).
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Chitinibacter,
Chitinibacter alvei Yang et al. 2010.
Gram-negative rods, 0.5-0.7 x 0.8-2.5 μm. Motile by a single polar flagellum.
Isolated from a stream located in southern Taiwan.
Sensitive to chloramphenicol, kanamycin, nalidixic acid, novobiocin, rifampicin, sulphamethoxazole, tetracycline, moderate to
streptomycin and resistant to ampicillin, gentamicin and penicillin G.
  1. Yang C.S., Sheu S.Y., Young C.C., Arun A.B., Cheng C.Y. and Chen W.M.: Chitinibacter alvei sp. nov., isolated from stream water.
    Int. J. Syst. Evol. Microbiol., 2010, 60, 1760-1764.
  2. Sheu, S. Y., LI, Y. S., Young, C. C. and Chen, W. M. 2016. Chitinibacter fontanus sp. nov., isolated from a spring. Int. J. Syst. Evol.
    Microbiol., 66, 4262-4268.
  3. Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
    1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature.
Most results are based on  API 20 NE, API ZYM and Biolog GN2.

Positive results for alkaline phosphatase, catalase, chitin hydrolysis (weak reaction), DNase, esculin hydrolysis, esterase (C4),
esterase lipase (C8), gelatin hydrolysis, alpha-glucosidase, N-acetyl-beta-glucosaminidase, leucine arylamidase, nitrate reduction,
oxidase, Tweens 20, 40 and 60 hydrolysis & acid production from glucose.
Utilizes as carbon substrates (Biolog GN2): dextrin, glycogen, Tween 40, Tween 80, N-acetyl-D-glucosamine, D-fructose,
alpha-D-glucose, maltose, D-mannose, pyruvic acid methyl ester, citric acid, D-gluconic acid, succinic acid, Lalaninamide, L-alanine,
L-alanyl glycine, L-asparagine, L-aspartic acid, L-glutamic acid, glycyl L-aspartic acid, glycyl L-glutamic acid, L-histidine,
L-phenylalanine, D-serine, L-serine & glycerol.

Negative results for acid phosphatase, arginine dihydrolase, casein hydrolysis, cystine arylamidase, alpha-fucosidase, alpha- and
beta-galactosidase, beta-glucuronidase, beta-glucosidase, indole production, lipase (corn oil), lipase (C14), alpha-mannosidase,
naphthol-AS-BI-phosphohydrolase, starch hydrolysis, Tween 80 hydrolysis, trypsin, alpha-chymotrypsin, urease & valine arylamidase.
No utilization of: citrate, alpha-cyclodextrin, N-acetyl-D-galactosamine, adonitol, D-arabitol, cellobiose, i-erythritol, L-fucose,
D-galactose, gentiobiose, myo-inositol, lactose, lactulose, D-mannitol, melibiose, methyl beta-D-glucoside, D-psicose, raffinose,
L-rhamnose, D-sorbitol, sucrose, trehalose, turanose, xylitol, succinic acid monomethyl ester, acetic acid, cis-aconitic acid, formic
acid, D-galactonic acid lactone, D-galacturonic acid, D-glucosaminic acid, D-glucuronic acid, alpha-, beta- and gamma-hydroxybutyric
acid, p-hydroxyphenylacetic acid, itaconic acid, alpha-ketobutyric acid, alpha-ketoglutaric acid, alpha-ketovaleric acid, DL-lactic acid,
malonic acid, propionic acid, quinic acid, D-saccharic acid, sebacic acid, bromosuccinic acid, succinamic acid, glucuronamide,
D-alanine, L-hydroxyproline, L-leucine, L-ornithine, L-proline, L-pyroglutamic acid, D-serine, DL-carnitine, gamma-aminobutyric acid,
urocanic acid, inosine, uridine, thymidine, phenylethylamine, putrescine, 2-aminoethanol, 2,3-butanediol, DL-alpha-glycerol
phosphate, alpha-D-glucose 1-phosphate & D-glucose 6-phosphate.
Chitinibacter alvei
(c) Costin Stoica
Culture media
Biochemical tests
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