Growth occurs at 03-38 ºC. Cannot grow anaerobically with nitrate. Grows in the
presence of 1% bile, but growth is inhibited by 1% glycine or 1% NaCl. Grows on EMB,
Seller and TSI agars. No growth on MacConkey agar or in MRVP broth. A fluorescent
pigment is produced. No pigment is produced from aromatic amino acids.
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Chromobacteriaceae, Genus Aquaspirillum,
Aquaspirillum metamorphum (Terasaki 1961) Hylemon et al. 1973.
Moved to genus Simplicispira as Simplicispira metamorpha (Terasaki 1961) Grabovich et al. 2006.
Old synonym: Spirillum metamorphum Terasaki 1961.
Gram-negative, helical cells, 1.1-1.3 / 4.0-11.0 μm. Motile by bipolar flagella.
Intracellular poly-beta-hydroxybutyrate is formed. Coccoid bodies are not formed.
Isolated from putrid infusion of freshwater shellfish.
- Hylemon (P.B.), Wells Jr. (J.S.), Krieg (N.R.) and Jannasch (H.W.): The genus Spirillum: A taxonomic study. International Journal of
Systematic Bacteriology, 1973, 23, 340-380.
- Tone Tonjum, 2005. Order IV. Neisseriales ord. nov. In: Bergey’s Manual of Systematic Bacteriology, Second edition, Vol two, part
C The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, George M. Garrity (Editor-in-Chief), pp 774-863.
- Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.,
1997, 47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
Positive results for catalase, gelatin liquefaction (within 4 days), H2S production from cysteine, oxidase, phosphatase, ribonuclease.
Can utilize succinate, fumarate, malate, oxaloacetate, pyruvate, lactate, L-alanine, L-glutamate, L-aspartate, L-glutamine &
Negative results for casein hydrolysis, DN-ase, esculin hydrolysis, hippurate hyrolysis, indole production, nitrate reduction, selenite
reduction, starch hydrolysis, urease. Acid is not produced from carbohydrates. Cannot utilize citrate, aconitate, isocitrate, caproate,
beta-hydroxybutyrate, p-hydroxybenzoate, ethanol, n-propanol, n-butanol, glycerol, D-fructose, D-glucose, D-xylose, L-arabinose,
L-histidine, L-tyrosine, L-phenylalanine, L-proline, L-hydroxyproline, L-ornithine, L-citrulline, L-arginine, L-lysine, putrescine,
L-methionine, L-serine, L-cysteine, glycine, L-leucine, L-isoleucine, L-valine & L-tryptophan.
Variable utilization of malonate, tartrate, acetate, propionate & butyrate.
(c) Costin Stoica