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Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus thermoamylovorans  Combet-Blanc,
Ollivier, Streicher, Patel, Dwivedi, Pot, Presnier and Garcia 1995.
Description is based on a single isolate.
Gram-positive, slightly motile rods, 0.45-0.5 x 3.0-4.0 µm, endospores  have not been
detected.
Colonies are white and lenticular, 2-3 mm in diameter / 2 days Facultatively
anaerobic. Methylotrophic, some strains also grow on ethanol. Moderately
thermophilic, growth temperature 50 - 55 ºC (optimum), pH 5.4-8.5 (optimum pH 7).
NaCl, alantoin or urate are not required for growth. Vitamins and nucleic acid
derivatives will stimulate growth, but are not essential.
Isolated from Senegalese palm wine, an African alcoholic bevarage. Cells are killed by heating at 80 ºC for 5 min.
Undetermined.
  1. N.A. Logan and P. De Vos, 2009. Genus I.  Bacillus  Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
    A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  2. Y. Combet-Blanc, B. Ollivier, C. Streicher, B. K. C. Patel, P. P. Dwivedi, B. Pot, G. Prensier,  and J.-L. Garcia, 1995. Bacillus
    themoamylovorans sp. nov., a Moderately Thermophilic and Amylolytic Bacterium.  IJSB 45, 1, 9-16.
Heterolactic fermentation of hexoses occurs; the end products are lactate, acetate,
ethanol, and formate but not H
2.

Positive results for arginine dihydrolase, catalase, hydrolysis of starch, acid production from: L-arabinose, D-glucose, D-mannose,
salicin, starch, D-xylose, N-acetyl-D-glucosamine, amygdalin, arbutin, cellobiose, D-fructose, D-galactose, beta-gentibiose,
gluconate, lactose, maltose, melezitose, rhamnose, sucrose & trehalose.

Negative results for oxidase, indole production, H
2S production, nitrate reduction, acid production from: D-mannitol, glycerol,
D-arabinose, D- or L-arabitol, erythritol, meso-inositol, inulin, lyxose, melibiose, raffinose, sorbose & xylitol.
Bacillus thermoamylovorans
(c) Costin Stoica
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