Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus tequilensis Gatson, Benz,
Chandrasekaran, Satomi, Venkateswaran and Hart 2006.
16S rRNA gene analysis demonstrated 99% similarity with B. subtilis.
Gram-positive, motile rods, 0.9 x 4.0 µm, single cells. Produce central endospores in
Colonies are smooth, circular, yellowish, up to 4 mm in diameter after 1 days at 37 ºC
on tryptone soy agar. Nonhemolytic on 5% sheep blood agar. Aerobic. No anaerobic
growth. Growth temperature 25 - 50 ºC, pH 5.5 - 8.
Isolated from an approximately 2000-year-old shaft-tomb at a site called Huitzilapa, near the city of Tequila in the Mexican state of
- Gatson J.W., Benz B.F., Chandrasekaran C., Satomi M., Venkateswaran K. and Hart M.E., 2006. Bacillus tequilensis sp. nov.,
isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis. IJSEM 56, 1475-1484.
Positive results for oxidase, catalase, arginine dihydrolase, beta-galactosidase, lysine
decarboxylase, ornithine decarboxylase, citrate utilization, hydrolysis of gelatin,
hydrolysis of casein, hydrolysis of starch, indole production, nitrate reduction,
tryptophan deaminase, Voges-Proskauer test, acid production from: L- or D-arabinose, adonitol, L- or D-arabitol, amygdalin, N-acetyl-
D-glucosamine, arbutin, cellobiose, dulcitol, fructose, L- or D-fucose, galactose, beta-gentiobiose, D-glucose, glycerol, glycogen,
meso-inositol, inulin, lactose, lyxose, maltose, D-mannitol, D-mannose, melibiose, raffinose, rhamnose, ribose, sorbitol, salicin,
sorbose, sucrose, starch, trehalose, xylitol, L- or D-xylose.
Negative results for hydrolysis of urea, acid production from: erythritol, gluconate, 2- or 5-ketogluconate, melezitose & methyl β-
Biochemically is very similar to B. subtilis from which can be differentiated by positive arginine dihydrolase, lysine decarboxylase,
ornithine decarboxylase and acid production from rhamnose.
(c) Costin Stoica