Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus subterraneus Kanso, Greene and
Patel 2002. Description is based upon a single isolate.
Gram-negative, nonspore-forming, motile by peritrichous flagella, curved rods, 0.5-0.8
x 2.0-25.0 µm. Occur singly, in pairs and chains.
Colonies are translucent, convex, with undulating irregular edges, 0.5-1.2 mm in
diameter after 2 days at 40 ºC on nutrient agar; on TSA they are dark yellow to orange,
mucoid and rhizoid. Facultatively anaerobic. Growth temperature 20 - 40 ºC
(optimum), pH 7-9. Grow in 0-9% NaCl. Alantoin or urate are not required for growth.
Utilizes amorphous iron (III), manganese (IV), nitrate, nitrite and fumarate as electron
acceptors in the presence of yeast extract, or certain carbohydrates, ethanol or lactate;
growth is better in the presence of nitrate. Yeast extract can be used as sole carbon
and energy source.
Isolated from deep subterranean waters of the Great Artesian Basin of Australia.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- S. Kanso, A. C. Greene and B. K. C. Patel, 2002. Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from
a deep subsurface Australian thermal aquifer. IJSEM 52, 869-874.
Positive results for hydrolysis of esculin, hydrolysis of gelatin, catalase, nitrate reduction & hydrolysis of starch.
Negative results for arginine dihydrolase, beta-galactosidase, lysine decarboxylase, ornithine decarboxylase, hydrolysis of urea,
oxidase, indole production, H2S production, hydrolysis of casein, Voges-Proskauer, tryptophan deaminase, acid production from:
L-arabinose, D-mannitol, D-mannose, adonitol, galactose, meso-inositol, melibiose, rhamnose, sorbitol & sucrose.
(c) Costin Stoica