Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Paenibacillaceae, Genus Paenibacillus, Paenibacillus sputi Kim, Lee, Yu,
Ryoo, Park and Lee, 2010.
Gram- variable, motile (polar flagella) rods, 0.5-0.6 x 2.0-3.0 µm. Spore is oval,
subterminally positioned, swelling the sporangium.
Colonies are colourless to white, translucent, mucoid on TSA. Alpha-haemolytic on
5% sheep blood agar. Aerobic and anerobic growth. Growth temperature 15-42 ºC,
optimum 30-37 ºC. Can grow in 0-3%, but not in 5% NaCl media. Optimum growth at
Isolated from human sputum. Susceptible to ampicillin, chloramphenicol, erythromycin, neomycin, penicillin G, rifampicin, streptomycin,
tetracycline, trimethoprim / sulfamethoxazole and vancomycin. Resistant to kanamycin.
The type strain was co-isolated from the sputum of a 60-year-old male patient during the screening of mycobacteria from patients with
nontuberculous pulmonary diseases.
- Kim, K.K., Lee , K.C., Yu, H., Ryoo, Y.P. and Lee, J.-S., 2010. Paenibacillus sputi sp. nov., isolated from the sputum of a patient with
pulmonary disease. IJSEM 60, 2371-2376.
Positive results for catalase, hydrolysis of esculin, beta-galactosidase, hydrolysis of
starch, hydrolysis of Tween 80, oxidase, Voges-Proskauer, xylanase, acid production
from: N-acetylglucosamine, aesculin, amygdalin, D- or L-arabinose, cellobiose,
L-fucose, galactose, D-glucose, methyl-alpha-D-glucoside, glycogen, lactose, maltose,
mannose, melibiose, raffinose, rhamnose, ribose, salicin, starch, D-xylose & methyl-beta-D-xyloside.
Negative results for arginine dihydrolase, DN-ase, H2S production, hydrolysis of casein, hydrolysis of gelatin, hydrolysis of urea,
indole production, lysine decarboxylase, methyl red, nitrate reduction, ornithine decarboxylase, phenylalanine deaminase,
degradation of tyrosine, acid production from: adonitol, arabitol, arbutin, dulcitol, erythritol, fructose, D-fucose, beta-gentiobiose,
gluconate, glycerol, inositol, inulin, 2- or 5-ketogluconate, lyxose, D-mannitol, methyl-alpha-D-mannoside, melezitose, sorbitol,
sorbose, sucrose, tagatose, trehalose, turanose, xylitol & L-xylose.
(c) Costin Stoica