Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus smithii Nakamura, Blumenstock &
Gram-positive rods, motile by peritrichous flagellation, 0.8-1.0 x 5.0-6.0 µm, producing
terminal/subterminal, ellipsoidal to cylindrical endospores (0.6-0.8 x 1.3-1.5 µm) in
Colonies are thin, smooth, translucent, unpigmented, and about 2 mm in diameter.
Good growth occurs in nutrient broth with 15% NaCl added.
Aerobic, facultatively anaerobic. Facultatively thermophilic. Growth temperature 25 - 65
ºC, pH 6-7. Grow in 2-3% NaCl, no growth in 5-10% NaCl. NaCl, alantoin or urate are
not required for growth.
Isolated from evaporated milk, canned foods, cheese, and sugar beet juice from extraction installations.
No growth in the presence of lysozyme.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- L.K. Nakamura, I. Blumenstock and D. Claus, 1988. Taxonomic Study of Bacillus coagulans Hammer 1915 with a Proposal for
Bacillus smithii sp. nov. IJSB 38, 63-73
Positive results for oxidase, catalase, hydrolysis of hippurate, hydrolysis of starch
(weak), acid production from: fructose, D-glucose, maltose & trehalose.
Negative results for arginine dihydrolase, degradation of tyrosine, deamination of phenylalanine, beta-galactosidase, lysine
decarboxylase, ornithine decarboxylase, hydrolysis of urea, hydrolysis of gelatin, egg yolk reaction, H2S production, indole production,
nitrate reduction, hydrolysis of casein, Voges-Proskauer test, acid production from: amygdalin, D-arabinose, D- or L-arabitol, arbutin,
adonitol, cellobiose, dulcitol, erythritol, D- or L-fucose, beta-gentibiose, gluconate, meso-inositol, inulin, 2- or 5-ketogluconate,
lactose, lyxose, methyl beta-xyloside, melezitose, melibiose, raffinose, N-acetyl-D-glucosamine, sorbose, sucrose, starch, xylitol & L-
Variable results for hydrolysis of esculin, citrate utilization, acid production from: L-arabinose, D-mannitol, D-mannose, galactose,
glycerol, rhamnose, ribose, sorbitol, salicin & D-xylose.
(c) Costin Stoica