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Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus siralis  Pettersson, de Silva, Uhlen
and Priest 2000.
Gram-positive rods, 0.5-0.8 x 2.0-3.0 µm. Produce subterminal / terminal, ellipsoidal
endospores in swollen sporangia.
Colonies are brown, circular, with shiny, glistening and granular surfaces, 3-5 mm in
diameter after 1 day on BHI agar. On nutrient agar the colonies are smaller (1-3 mm
in diameter), pale and opaque. Aerobic, no anaerobic growth.
Growth parameteres: maximum temperature 50 ºC, pH 7, 0-7% NaCl. Alantoin or
urate are not required for growth.
Isolated from silage by heat treating the sample at 100 °C for 60 min and plating onto brain heart infusion agar.
Resistant to erythromycin (5 µg / ml).
Undetermined.
  1. N.A. Logan and P. De Vos, 2009. Genus I.  Bacillus  Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
    A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  2. B. Pettersson, S.K. de Silva, M. Uhlen and F. G. Priest, 2000. Bacillus siralis sp. nov., a novel species from silage with a higher
    order structural attribute in the 16S rRNA genes. IJSEM 50, 2181-2187.
Positive results for hydrolysis of esculin, hydrolysis of gelatin, oxidase, catalase,
nitrate reduction & hydrolysis of casein.

Negative results for degradation of tyrosine, deamination of phenylalanine, hydrolysis of starch, Voges-Proskauer, citrateutilization,
tryptophan deaminase, acid production from: L- or D-arabinose, D-glucose, D-mannitol, D-mannose, glycerol, salicin, cellobiose,
fructose, galactose, meso-inositol, lactose, maltose, melibiose, raffinose, rhamnose, ribose, sorbitol, sucrose, trehalose, L- or
D-xylose.

Variable results for arginine dihydrolase.
Bacillus siralis
(c) Costin Stoica
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