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Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus siralis  Pettersson, de Silva, Uhlen
and Priest 2000.
Gram-positive rods, 0.5-0.8 x 2.0-3.0 µm. Produce subterminal / terminal, ellipsoidal
endospores in swollen sporangia.
Colonies are brown, circular, with shiny, glistening and granular surfaces, 3-5 mm in
diameter after 1 day on BHI agar. On nutrient agar the colonies are smaller (1-3 mm
in diameter), pale and opaque. Aerobic, no anaerobic growth.
Growth parameteres: maximum temperature 50 ºC, pH 7, 0-7% NaCl. Alantoin or
urate are not required for growth.
Isolated from silage by heat treating the sample at 100 ºC for 60 min and plating onto Brain heart infusion agar.
Resistant to erythromycin (5 µg / ml).
Undetermined.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
    Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  2. B. Pettersson, S.K. de Silva, M. Uhlen and F. G. Priest, 2000. Bacillus siralis sp. nov., a novel species from silage with a higher
    order structural attribute in the 16S rRNA genes. IJSEM 50, 2181-2187.
Positive results for hydrolysis of esculin, hydrolysis of gelatin, oxidase, catalase,
nitrate reduction & hydrolysis of casein.

Negative results for degradation of tyrosine, deamination of phenylalanine, hydrolysis of starch, Voges-Proskauer, citrate utilization,
tryptophan deaminase, acid production from: L- or D-arabinose, D-glucose, D-mannitol, D-mannose, glycerol, salicin, cellobiose,
fructose, galactose, meso-inositol, lactose, maltose, melibiose, raffinose, rhamnose, ribose, sorbitol, sucrose, trehalose, L- or
D-xylose.

Variable results for arginine dihydrolase.
Bacillus siralis
(c) Costin Stoica
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